Department of Biology, Plant Biotechnology, ETH Zurich-LFW E56,1, Universitaetstrasse 2, 8092, Zurich, Switzerland.
Plant Methods. 2012 Jul 11;8(1):24. doi: 10.1186/1746-4811-8-24.
Recent progress in cassava transformation has allowed the robust production of transgenic cassava even under suboptimal plant tissue culture conditions. The transformation protocol has so far been used mostly for the cassava model cultivar 60444 because of its good regeneration capacity of embryogenic tissues. However, for deployment and adoption of transgenic cassava in the field it is important to develop robust transformation methods for farmer- and industry-preferred landraces and cultivars. Because dynamics of multiplication and regeneration of embryogenic tissues differ between cassava genotypes, it was necessary to adapt the efficient cv. 60444 transformation protocol to genotypes that are more recalcitrant to transformation. Here we demonstrate that an improved cassava transformation protocol for cv. 60444 could be successfully modified for production of transgenic farmer-preferred cassava landraces. The modified transformation method reports on procedures for optimization and is likely transferable to other cassava genotypes reportedly recalcitrant to transformation provided production of high quality FEC. Because the three farmer-preferred cassava landraces selected in this study have been identified as resistant or tolerant to cassava mosaic disease (CMD), the adapted protocol will be essential to mobilize improved traits into cassava genotypes suitable for regions where CMD limits production.
最近，木薯转化方面的进展使得即使在植物组织培养条件不佳的情况下，也能够大量生产转基因木薯。由于其胚胎组织良好的再生能力，该转化方案迄今为止主要用于木薯模式品种 60444。然而，为了在田间推广和采用转基因木薯，开发针对农民和工业首选的地方品种和栽培品种的稳健转化方法非常重要。由于胚胎组织的增殖和再生动力学在不同木薯基因型之间存在差异，因此有必要对更难以转化的基因型进行适应性调整。在这里，我们证明了可以成功地将针对 cv.60444 的改良木薯转化方案进行修改，以生产转基因农民首选的木薯地方品种。该改良的转化方法报告了优化程序，并且可能适用于其他据报道对转化具有抗性的木薯基因型，只要能够生产出高质量的 FEC。由于本研究中选择的三个农民首选的木薯地方品种被鉴定为对木薯花叶病（CMD）具有抗性或耐受性，因此，该适应的方案对于将改良的性状转移到适合 CMD 限制生产的地区的木薯基因型中至关重要。
