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采用高效液相色谱-二极管阵列检测-串联质谱(HPLC-DAD-MS(n))法测定巴西可可(Oenocarpus bacaba Mart.)果实中酚类化合物的抗氧化活性及特性。

Antioxidant activity and characterization of phenolic compounds from bacaba (Oenocarpus bacaba Mart.) fruit by HPLC-DAD-MS(n).

机构信息

Institute of Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany.

出版信息

J Agric Food Chem. 2012 Aug 8;60(31):7665-73. doi: 10.1021/jf3007689. Epub 2012 Jul 26.

Abstract

The phytochemicals in fruits have been shown to be major bioactive compounds with regard to health benefits. Bacaba (Oenocarpus bacaba Mart.) is a native palm fruit from the Brazilian savannah and Amazon rainforest that plays an important role in the diet of rural communities and is also a source of income for poor people. This paper reports the characterization and analyses of phenolics from bacaba fruit extract. The total phenolic content of bacaba fruit amounted to 1759.27 ± 1.01 mg GAE/100 g, the flavonoid content was 1134.32 ± 0.03 mg CTE/100 g, and the anthocyanin content was 34.69 ± 0.00 mg cyn-3-glc/100 g. The antioxidant activity was evaluated through different assays [ORAC, FRAP, DPPH, TEAC, and cellular antioxidant assay (CAA) assays] and revealed a significant antioxidant capacity for bacaba in comparison to the data available in the literature. The assignment of the phenolic compounds using HPLC-DAD-MS(n) was based on the evaluation of their UV-vis absorption maxima (λ(max)) and mass spectral analyses, and 14 compounds were tentatively identified. The results suggest that bacaba fruits are a promising source of phenolics.

摘要

水果中的植物化学物质已被证明是具有健康益处的主要生物活性化合物。巴西大蕉(Oenocarpus bacaba Mart.)是一种原产于巴西稀树草原和亚马逊雨林的棕榈水果,在农村社区的饮食中起着重要作用,也是贫困人口的收入来源。本文报道了巴西大蕉果实提取物中酚类物质的特征和分析。巴西大蕉果实的总酚含量为 1759.27 ± 1.01 mg GAE/100 g,类黄酮含量为 1134.32 ± 0.03 mg CTE/100 g,花色苷含量为 34.69 ± 0.00 mg cyn-3-glc/100 g。通过不同的测定方法[ORAC、FRAP、DPPH、TEAC 和细胞抗氧化测定(CAA)]评估了抗氧化活性,结果表明巴西大蕉的抗氧化能力与文献中可用的数据相比具有显著的抗氧化能力。使用 HPLC-DAD-MS(n) 对酚类化合物进行归属,基于对其紫外可见吸收最大值(λ(max))和质谱分析的评估,初步鉴定了 14 种化合物。结果表明,巴西大蕉果实是酚类物质的有前途的来源。

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