Pharmacognosy Laboratory, Faculty of Pharmaceutical Sciences, Shoolini University, Solan 173212, Himachal Pradesh, India.
J Ethnopharmacol. 2012 Aug 30;143(1):194-200. doi: 10.1016/j.jep.2012.06.023. Epub 2012 Jul 10.
Tecomella undulata (TU;` Family Bignoniaceae) is used in Indian Ayurvedic system of medicine for treating various diseases including hepatic ailments. It is also incorporated in various marketed hepatoprotective polyherbal formulations.
The present study was aimed at evaluating possible hepatoprotective role of isolated compounds from TU stem bark (TSB) using in vitro and in vivo experimental models.
In vitro cytotoxicity and hepatoprotective potential of various extract, fractions and isolated compounds from TU stem bark were evaluated using HepG2 cells. Rats were pre-treated with TU methanolic extract (TSB-7) or betulinic acid (MS-2) or silymarin for 7 days followed by a single dose of CCl(4) (0.5 ml/kg, i.p.). Plasma markers of hepatic damage, hepatic antioxidants and indices of lipid peroxidation along with microscopic evaluation of liver were assessed in control and treatment groups.
TSB-2 and MS-1 accounted for significant cell death whereas; TSB-1, TBS-7, TSB-9, TSB-10 and, MS-2 did not register significant cytotoxicity. Further, non-cytotoxic components exhibited ascending grade of hepatoprotection in vitro (TSB-10<TSB-1<TSB-7<TSB-9<MS-2). Pre-treatment of TSB-7 or MS-2 to CCl(4) treated rats prevented hepatocyte damage as evidenced by biochemical and histopathological observations.
It can be concluded that, hepatoprotective potential of Tecomella undulata stem bark is partially due to the presence of betulinic acid.
Tecomella undulata（TU；家族紫葳科）在印度阿育吠陀医学体系中用于治疗各种疾病，包括肝脏疾病。它也被纳入各种市售的保肝复方草药制剂中。
本研究旨在评估 TU 茎皮中分离化合物的体外和体内实验模型的潜在保肝作用。
采用 HepG2 细胞评估 TU 茎皮的各种提取物、馏分和分离化合物的体外细胞毒性和保肝潜力。大鼠用 TU 甲醇提取物（TSB-7）或白桦脂酸（MS-2）或水飞蓟素预处理 7 天，然后单次腹腔注射 CCl4（0.5ml/kg）。在对照组和治疗组中评估血浆肝损伤标志物、肝抗氧化剂和脂质过氧化指标以及肝的显微镜评估。
TSB-2 和 MS-1 导致显著的细胞死亡，而 TSB-1、TSB-7、TSB-9、TSB-10 和 MS-2 未显示出显著的细胞毒性。此外，非细胞毒性成分在体外表现出递增的保肝作用（TSB-10<TSB-1<TSB-7<TSB-9<MS-2）。TSB-7 或 MS-2 预处理 CCl4 处理大鼠可预防肝细胞损伤，这可通过生化和组织病理学观察得到证明。
可以得出结论，Tecomella undulata 茎皮的保肝潜力部分归因于白桦脂酸的存在。
