Ito Tetsuro, Kakino Mamoru, Tazawa Shigemi, Watarai Tatsuya, Oyama Masayoshi, Maruyama Hiroe, Araki Yoko, Hara Hideaki, Iinuma Munekazu
Laboratory of Pharmacognosy, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
J Nutr Sci Vitaminol (Tokyo). 2012;58(2):136-42. doi: 10.3177/jnsv.58.136.
Mangiferin (3) and genkwanin 5-O-β-primeveroside (5) are the two major bioactive polyphenols with laxative property present in the extracts of agarwood (Aquilaria sinensis) leaves (AL). Here we developed an HPLC method to determine these bioactive components and four other major polyphenols in AL extracts and evaluated the pharmacological equivalence of organic and water extracts. Using mobile phase gradient conditions combined with UV detection at 330 nm, all six compounds were separated and we determined the relative extraction ratios of the six compounds present in A. sinensis extracts that were prepared under different conditions and compared the contents of the two laxative polyphenols present in the 60% ethanol extracts of A. sinensis and A. crassna. The polyphenols present in water extracts of 13 commercially cultivated A. crassna plants have also been analyzed. The laxative properties of 60% ethanol and four water extracts of A. crassna were evaluated by the frequency and weight of stools in loperamide-induced constipation model mice. The pharmacological equivalence of 60% ethanol extract and hot water (95°C) extract was identified in mice.
芒果苷（3）和芫花素5-O-β-樱草糖苷（5）是沉香（Aquilaria sinensis）叶提取物中存在的两种具有通便特性的主要生物活性多酚。在此，我们开发了一种高效液相色谱法来测定沉香叶提取物中的这些生物活性成分以及其他四种主要多酚，并评估了有机提取物和水提取物的药理等效性。采用流动相梯度条件并结合330 nm紫外检测，分离出了所有六种化合物，我们测定了在不同条件下制备的沉香提取物中六种化合物的相对提取率，并比较了沉香和厚叶沉香60%乙醇提取物中两种具有通便作用的多酚的含量。还分析了13种商业种植的厚叶沉香植物水提取物中的多酚。通过洛哌丁胺诱导的便秘模型小鼠的粪便频率和重量，评估了厚叶沉香60%乙醇提取物和四种水提取物的通便特性。在小鼠中确定了60%乙醇提取物和热水（95°C）提取物的药理等效性。
