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利用荧光光谱成像技术定量测定沉香形成比率

Measuring Agarwood Formation Ratio Quantitatively by Fluorescence Spectral Imaging Technique.

作者信息

Huang Botao, Nguyen Duykien, Liu Tianyi, Jiang Kaibin, Tan Jinfen, Liu Chunxin, Zhao Jing, Huang Shaowei

机构信息

College of Science, South China Agricultural University, Guangzhou, Guangdong 510640, China.

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, Guangdong 510640, China ; College of Forestry, South China Agricultural University, Guangzhou, Guangdong 510640, China.

出版信息

Evid Based Complement Alternat Med. 2015;2015:205089. doi: 10.1155/2015/205089. Epub 2015 May 18.

DOI:10.1155/2015/205089
PMID:26089935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4451979/
Abstract

Agarwood is a kind of important and precious traditional Chinese medicine. With the decreasing of natural agarwood, artificial cultivation has become more and more important in recent years. Quantifying the formation of agarwood is an essential work which could provide information for guiding cultivation and controlling quality. But people only can judge the amount of agarwood qualitatively by experience before. Fluorescence multispectral imaging method is presented to measure the agarwood quantitatively in this paper. A spectral cube from 450 nm to 800 nm was captured under the 365 nm excitation sources. The nonagarwood, agarwood, and rotten wood in the same sample were distinguished based on analyzing the spectral cube. Then the area ratio of agarwood to the whole sample was worked out, which is the quantitative information of agarwood area percentage. To our knowledge, this is the first time that the formation of agarwood was quantified accurately and nondestructively.

摘要

沉香是一种重要且珍贵的传统中药。随着天然沉香数量的减少,近年来人工种植变得越来越重要。量化沉香的形成是一项至关重要的工作,可为指导种植和控制质量提供信息。但此前人们只能凭经验对沉香的数量进行定性判断。本文提出采用荧光多光谱成像方法对沉香进行定量测量。在365nm激发光源下采集了450nm至800nm的光谱立方体。通过对光谱立方体进行分析,区分了同一样本中的非沉香、沉香和腐朽木材。然后计算出沉香占整个样本的面积比,即沉香面积百分比的定量信息。据我们所知,这是首次对沉香的形成进行准确且无损的量化。

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引用本文的文献

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本文引用的文献

1
Distinguishing wild from cultivated agarwood (Aquilaria spp.) using direct analysis in real time and time of-flight mass spectrometry.利用实时直接分析和飞行时间质谱法鉴别野生和栽培沉香(Aquilaria spp.)。
Rapid Commun Mass Spectrom. 2014 Feb 15;28(3):281-9. doi: 10.1002/rcm.6779.
2
Whole-tree agarwood-inducing technique: an efficient novel technique for producing high-quality agarwood in cultivated Aquilaria sinensis trees.整树结香技术:一种高效的新型技术,可用于生产高质量人工种植沉香树的沉香。
Molecules. 2013 Mar 7;18(3):3086-106. doi: 10.3390/molecules18033086.
3
Quantification of polyphenols and pharmacological analysis of water and ethanol-based extracts of cultivated agarwood leaves.
人工种植沉香叶中多酚的定量分析以及水提取物和乙醇提取物的药理分析
J Nutr Sci Vitaminol (Tokyo). 2012;58(2):136-42. doi: 10.3177/jnsv.58.136.
4
[The research on active constituent distribution of rhizoma coptidis pieces].[黄连饮片活性成分分布研究]
Guang Pu Xue Yu Guang Pu Fen Xi. 2011 Jun;31(6):1692-6.
5
Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis.沉香药用部位内生真菌的抗肿瘤和抗菌活性。
J Zhejiang Univ Sci B. 2011 May;12(5):385-92. doi: 10.1631/jzus.B1000330.
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[In vivo identification of radix panacis quinquefolii by spectral imaging technology].[基于光谱成像技术的西洋参活体鉴别]
Guang Pu Xue Yu Guang Pu Fen Xi. 2011 Jan;31(1):210-3.
7
Laxative effects of agarwood on low-fiber diet-induced constipation in rats.沉香木对低纤维饮食诱导的大鼠便秘的通便作用。
BMC Complement Altern Med. 2010 Nov 15;10:68. doi: 10.1186/1472-6882-10-68.
8
Agarwood induced laxative effects via acetylcholine receptors on loperamide-induced constipation in mice.沉香通过乙酰胆碱受体对洛哌丁胺诱导的小鼠便秘产生通便作用。
Biosci Biotechnol Biochem. 2010;74(8):1550-5. doi: 10.1271/bbb.100122. Epub 2010 Aug 7.
9
Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components.沉香木油与甘松提取物蒸汽吸入的镇静作用及其活性成分鉴定
J Nat Med. 2008 Jan;62(1):41-6. doi: 10.1007/s11418-007-0177-0. Epub 2007 Aug 23.
10
New sesquiterpene from Vietnamese agarwood and its induction effect on brain-derived neurotrophic factor mRNA expression in vitro.来自越南沉香的新型倍半萜及其对体外脑源性神经营养因子mRNA表达的诱导作用。
Bioorg Med Chem. 2006 May 15;14(10):3571-4. doi: 10.1016/j.bmc.2006.01.023. Epub 2006 Feb 7.