头颈部鳞状细胞癌中的病毒载量、身体状况和人乳头瘤病毒 E6/E7 mRNA 表达。

Viral load, physical status, and E6/E7 mRNA expression of human papillomavirus in head and neck squamous cell carcinoma.

机构信息

Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.

出版信息

Head Neck. 2013 Jun;35(6):800-8. doi: 10.1002/hed.23034. Epub 2012 Jul 13.

DOI:10.1002/hed.23034

PMID:22791649

Abstract

BACKGROUND

The purpose of this study was to determine prospectively both human papillomavirus (HPV) load and physical status in different types of head and neck squamous cell carcinoma (HNSCC).

METHODS

HPV DNA, E6/E7 mRNA expression, viral load, and physical status of 184 patients with HNSCC were examined simultaneously by polymerase chain reaction (PCR)-based methods.

RESULTS

The HPV genome was detected in 54 HNSCC samples (29.3%), particularly in tonsillar carcinomas (69.6%). Compared with nonoropharyngeal HNSCC, oropharyngeal carcinoma harbored a relatively higher viral load, especially in tonsillar carcinoma. Although integrated or mixed status was observed in 75.6% of HPV-16-positive samples, E6/E7 mRNA transcripts were detected in only 27.5% of HPV DNA-positive cases. High HPV-16 load correlated significantly with E6/E7 mRNA expression.

CONCLUSION

E6/E7 mRNA expression in patients with HNSCC with low viral load remains low even in cases of integration to the host genome. Tonsillar carcinomas were significantly associated with HPV among various types of HNSCC.

摘要

背景

本研究旨在前瞻性地确定不同类型头颈部鳞状细胞癌（HNSCC）中的人乳头瘤病毒（HPV）载量和物理状态。

方法

通过聚合酶链反应（PCR）方法同时检测了 184 例 HNSCC 患者的 HPV DNA、E6/E7 mRNA 表达、病毒载量和物理状态。

结果

在 54 例 HNSCC 样本（29.3%）中检测到 HPV 基因组，特别是在扁桃体癌（69.6%）中。与非口咽 HNSCC 相比，口咽癌携带相对较高的病毒载量，特别是在扁桃体癌中。尽管在 75.6%的 HPV-16 阳性样本中观察到整合或混合状态，但仅在 27.5%的 HPV DNA 阳性病例中检测到 E6/E7 mRNA 转录本。高 HPV-16 载量与 E6/E7 mRNA 表达显著相关。