School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
J Zhejiang Univ Sci B. 2020;21(12):961-976. doi: 10.1631/jzus.B2000161.
Worldwide there has been a significant increase in the incidence of oropharyngeal squamous cell carcinoma (OPSCC) etiologically attributed to oncogenic human papillomavirus (HPV). Reliable and accurate identification and detection tools are important as the incidence of HPV-related cancer is on the rise. Several HPV detection methods for OPSCC have been developed and each has its own advantages and disadvantages in regard to sensitivity, specificity, and technical difficulty. This review summarizes our current knowledge of molecular methods for detecting HPV in OPSCC, including HPV DNA/RNA polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), p16 immunohistochemistry (IHC), and DNA/RNA in situ hybridization (ISH) assays. This summary may facilitate the selection of a suitable method for detecting HPV infection, and therefore may help in the early diagnosis of HPV-related carcinoma to reduce its mortality, incidence, and morbidity.
全球范围内，与致癌型人乳头瘤病毒（HPV）相关的口咽鳞状细胞癌（OPSCC）发病率显著增加。可靠且准确的识别和检测工具非常重要，因为 HPV 相关癌症的发病率正在上升。已经开发出几种用于 OPSCC 的 HPV 检测方法，每种方法在灵敏度、特异性和技术难度方面都有其自身的优势和劣势。本文综述了用于检测 OPSCC 中 HPV 的分子方法的现有知识，包括 HPV DNA/RNA 聚合酶链反应（PCR）、环介导等温扩增（LAMP）、p16 免疫组织化学（IHC）和 DNA/RNA 原位杂交（ISH）检测。本文综述可能有助于选择合适的 HPV 感染检测方法，从而有助于早期诊断 HPV 相关癌，降低其死亡率、发病率和患病率。
