Ecole Nationale d'Ingénieurs de Saint Etienne (ENISE), LTDS, UMR 5513 CNRS, Université de Lyon, 42023 Saint-Etienne, France.
J Biomed Mater Res A. 2012 Nov;100(11):3108-16. doi: 10.1002/jbm.a.34239. Epub 2012 Jul 13.
The femtosecond laser processing enabled the structuring of six types of surfaces on titanium-6aluminium-4vanadium (Ti-6Al-4V) plates. The obtained hierarchical features consisted of a combination of microgrooves and oriented nanostructures. By adjusting beam properties such as laser polarization, the width of the microgrooves (20 or 60 μm) and the orientation of the nanostructures (parallel or perpendicular to the microgrooves) can be precisely controlled. Mesenchymal stem cells (MSCs) grown on these structured surfaces produced cytoplasmic extensions with focal contacts, while on the smooth titanium, the cells were found to be well spread and without any focal contact 12 h postseeding. The 600-nm wide nanostructures on their own were sufficient to orient the MSCs. For the multiscale structured areas, when the orientation of the nanostructures was orthogonal in relation to the microgrooves, there was an important decrease in or even a loss of cell alignment signifying that cells were sensitive to the directional nanostructures in the microgrooves. At 7 days, cell proliferation was not affected but the direction of nanostructures controlled the matrix organization. The ultrafast laser, as a new method for producing micro-nanohybrid surfaces, is a promising approach to promote desired tissue organization for tissue engineering.
飞秒激光处理使钛-6 铝-4 钒（Ti-6Al-4V）板的六种表面结构得以实现。所获得的分层特征由微槽和定向纳米结构的组合组成。通过调整光束特性，如激光偏振，可以精确控制微槽的宽度（20 或 60μm）和纳米结构的取向（平行或垂直于微槽）。在这些结构化表面上生长的间充质干细胞（MSCs）产生了具有焦点接触的细胞质延伸，而在光滑的钛上，发现细胞在接种后 12 小时铺展良好且没有任何焦点接触。600nm 宽的纳米结构本身足以定向 MSCs。对于多尺度结构化区域，当纳米结构的取向与微槽正交时，细胞排列的重要性降低甚至丧失，这表明细胞对微槽中的定向纳米结构敏感。在 7 天内，细胞增殖不受影响，但纳米结构的方向控制着基质组织。超短激光作为一种制造微纳混合表面的新方法，是一种很有前途的方法，可以促进组织工程中所需的组织组织。
