College of Mechanical and Electrical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, CAS, Xi'an 710119, China.
Molecules. 2020 Mar 25;25(7):1494. doi: 10.3390/molecules25071494.
The bioactivity and biocompatibility play key roles in the success of dental and orthopaedic implants. Although most commercial implant systems use various surface microstructures, the ideal multi-scale topographies capable of controlling osteointegration have not yielded conclusive results. Inspired by both the isotropic adhesion of the skin structures in tree frog toe pads and the anisotropic adhesion of the corrugated ridges on the scales of Morpho butterfly wings, composite micro/nano-structures, including the array of micro-hexagons and oriented nano-ripples on titanium alloy implants, were respectively fabricated by microsecond laser direct writing and femtosecond laser-induced periodic surface structures, to improve cell adherence, alignment and proliferation on implants. The main differences in both the bioactivity in simulated body fluid and the biocompatibility in osteoblastic cell MC3T3 proliferation were measured and analyzed among Ti-6Al-4V samples with smooth surface, micro-hexagons and composite micro/nano-structures, respectively. Of note, bioinspired micro/nano-structures displayed the best bioactivity and biocompatibility after in vitro experiments, and meanwhile, the nano-ripples were able to induce cellular alignment within the micro-hexagons. The reasons for these differences were found in the topographical cues. An innovative functionalization strategy of controlling the osteointegration on titanium alloy implants is proposed using the composite micro/nano-structures, which is meaningful in various regenerative medicine applications and implant fields.
生物活性和生物相容性在牙科和骨科植入物的成功中起着关键作用。尽管大多数商业植入系统使用各种表面微观结构，但能够控制骨整合的理想多尺度形貌尚未得出明确的结果。受树蛙脚趾垫的各向同性粘附力和 Morpho 蝴蝶翅膀上的波纹脊的各向异性粘附力的启发，分别通过微秒激光直写和飞秒激光诱导的周期性表面结构在钛合金植入物上制造了复合微/纳米结构，包括微六边形阵列和定向纳米波纹，以提高细胞在植入物上的粘附、排列和增殖。在模拟体液中的生物活性和成骨细胞 MC3T3 增殖中的生物相容性方面，分别测量和分析了具有光滑表面、微六边形和复合微/纳米结构的 Ti-6Al-4V 样品之间的主要差异。值得注意的是，经过体外实验，仿生微/纳米结构显示出最佳的生物活性和生物相容性，同时，纳米波纹能够诱导微六边形内的细胞排列。这些差异的原因在于形貌线索。提出了一种使用复合微/纳米结构控制钛合金植入物骨整合的创新功能化策略，这在各种再生医学应用和植入物领域具有重要意义。
