聚茴香脑磺酸钠和淀粉硫酸酯钠对新鲜去纤维蛋白人血中几种非特异性抗菌系统的可变中和作用

Variable neutralization of several nonspecific antibacterial systems in fresh, defibrinated human blood by sodium polyanetholsulfonate and sodium amylosulfate.

作者信息

Traub W H, Fukushima P I

出版信息

J Clin Microbiol. 1979 Jul;10(1):27-31. doi: 10.1128/jcm.10.1.27-31.1979.

DOI:10.1128/jcm.10.1.27-31.1979

PMID:227919

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273087/

Abstract

Fresh, defibrinated human blood (80 vol%, i.e., 80% [vol/vol] of a 2-ml final assay volume) from two healthy adult donors killed "delayed serum-sensitive" (DSS) and "promptly serum-sensitive" (PSS) strains of Serratia marcescens, PSS control strain Escherichia coli C, Bacillus subtilis strain ATCC 6633, and Micrococcus lysodeikticus ATCC 4698 in a kinetic manner comparable to that of fresh human serum (80 vol%). However, heat-inactivated (56 degrees C, 30 min), defibrinated human blood revealed markedly reduced or a total lack of beta-lysin activity against the B. subtilis assay strain. Similarly, lysozyme activity of defibrinated blood was diminished somewhat by heat treatment, as determined with the M. lysodeikticus assay strain. Addition of 500 mug of sodium polyanetholsulfonate (SPS) per ml to 80 vol% of fresh, defibrinated human blood completely neutralized blood bactericidal activity against all assay strains of S. marcescens, E. coli C, and B. subtilis; however, SPS at this concentration failed to abolish lysozyme activity for prolonged periods of incubation. Addition of 500 mug of sodium amylosulfate (SAS) per ml to 80 vol% of fresh defibtinated human blood resulted in protection of cell inocula of DSS strains of S. marcescens only; SAS failed to protect cell inocula of the PSS strains of S. marcescens, E. coli C, B. subtilis, and M. lysodeikticus for extended periods of observation. Based on these data, it is recommended that blood culture specimens that are first drawn into specimen containers (such as Vacutainer tubes or the like) at the patient's bedside, and which contain >/=250 mug of SPS per ml, be diluted into suitable broth media with at least >/=250 mug of SPS per ml by the receiving laboratory within 2 to 4 h after procurement of the specimen. This procedure would ensure continued, adequate neutralization of the specimen's inherent beta-lysin, lysozyme, and complement- and antibody-mediated bactericidal activities.

摘要

来自两名健康成年献血者的新鲜、去纤维蛋白人血（80体积%，即2毫升最终测定体积的80%[体积/体积]）以与新鲜人血清（80体积%）相当的动力学方式杀死了粘质沙雷氏菌的“延迟血清敏感”（DSS）和“快速血清敏感”（PSS）菌株、PSS对照菌株大肠杆菌C、枯草芽孢杆菌ATCC 6633菌株以及溶壁微球菌ATCC 4698。然而，热灭活（56℃，30分钟）的去纤维蛋白人血对枯草芽孢杆菌测定菌株的β-溶素活性明显降低或完全缺乏。同样，用溶壁微球菌测定菌株测定发现，去纤维蛋白血的溶菌酶活性经热处理后有所降低。每毫升80体积%的新鲜、去纤维蛋白人血中添加500微克多聚茴香脑磺酸钠（SPS）可完全中和血液对所有粘质沙雷氏菌、大肠杆菌C和枯草芽孢杆菌测定菌株的杀菌活性；然而，在此浓度下，SPS在长时间孵育时未能消除溶菌酶活性。每毫升80体积%的新鲜去纤维蛋白人血中添加500微克淀粉硫酸酯钠（SAS）仅能保护粘质沙雷氏菌DSS菌株的细胞接种物；在延长的观察期内，SAS未能保护粘质沙雷氏菌PSS菌株、大肠杆菌C、枯草芽孢杆菌和溶壁微球菌的细胞接种物。基于这些数据，建议在患者床边首先采集到标本容器（如真空采血管等）中且每毫升含有≥250微克SPS的血培养标本，在标本采集后2至4小时内由接收实验室稀释到每毫升至少含有≥250微克SPS的合适肉汤培养基中。此程序将确保持续、充分地中和标本固有的β-溶素、溶菌酶以及补体和抗体介导的杀菌活性。