粘质沙雷氏菌对哺乳动物血清的敏感性：三种人血清敏感性类别的检测

Mammalian serum susceptibility of Serratia marcescens: detection of three human serum susceptibility categories.

作者信息

Traub W H, Fukushima P I

出版信息

Zentralbl Bakteriol Orig A. 1979;245(3):301-11.

Abstract

A total of 65 strains of S. marcescens, including 36 currently employed O- and H-antigen reference strains, were examined for their susceptibility to the bactericidal activity of 80 vol % of fresh human serum. The majority of strains (57 = 87.6%) proved 'delayed serum-sensitive' (DSS); 4 strains (6.2%) were 'promptly serum-sensitive' (PSS), whereas 4 strains (6.2%) resisted (NSS) complement-mediated killing by human serum. Fresh sera obtained from 7 adult human volunteers yielded essentially identical kinetic killing data against representative NSS, DSS and PSS strains of S. marcescens. These observations were interpreted as further justification for the assignment of clinical isolates of S. marcescens to 3 categories of human serum susceptibility: DSS, PSS and NSS. Species differences among fresh sera from sheep, rabbits and guinea pigs, as contrasted with fresh human serum, were noted. In general, PSS strains of S. marcescens were killed in a slightly delayed fashion; DSS strains of S. marcescens were killed in an unpredictable kinetic pattern by sheep and rabbit sera, whereas fresh guinea pig serum entirely failed to kill selected DSS isolates of S. marcescens.

摘要

共检测了65株粘质沙雷氏菌，其中包括36株目前使用的O抗原和H抗原参考菌株，以检测它们对80%体积分数的新鲜人血清杀菌活性的敏感性。大多数菌株（57株，占87.6%）被证明为“延迟血清敏感型”（DSS）；4株（占6.2%）为“快速血清敏感型”（PSS），而4株（占6.2%）对人血清补体介导的杀伤具有抗性（NSS）。从7名成年人类志愿者获得的新鲜血清对粘质沙雷氏菌的代表性NSS、DSS和PSS菌株产生了基本相同的动力学杀伤数据。这些观察结果被解释为将粘质沙雷氏菌临床分离株分为3类人血清敏感性（DSS、PSS和NSS）的进一步依据。与新鲜人血清相比，注意到绵羊、兔子和豚鼠新鲜血清之间的种属差异。一般来说，粘质沙雷氏菌的PSS菌株被杀灭的时间稍有延迟；粘质沙雷氏菌的DSS菌株被绵羊和兔子血清以不可预测的动力学模式杀灭，而新鲜豚鼠血清完全无法杀灭选定的粘质沙雷氏菌DSS分离株。