Traub W H
J Clin Microbiol. 1977 Aug;6(2):128-31. doi: 10.1128/jcm.6.2.128-131.1977.
The synthetic anticoagulant sodium amylosulfate (SAS) at concentrations of 125 to 2,000 microgram/ml failed to completely neutralize the bactericidal activity of 80 and 50% (by volume) fresh human serum. Furthermore, SAS failed to inhibit the alternative pathway of complement activation in 80% (by volume) fresh human serum that had been chelated with 0.01 M magnesium ions plus 0.01 M ethyleneglycol-bis(beta-aminoethylether)-N,N-tetraacetic acid. However, SAS at 250 to 1,000 microgram/ml effectively neutralized the bactericidal activity of 20% (by volume) fresh human serum. Therefore, SAS (at 250 to 1,000 microgram/ml) should be used only in blood samples that have been diluted at least fivefold (less than or equal to 20% [by volume]) in suitable broth media.
浓度为125至2000微克/毫升的合成抗凝剂淀粉硫酸酯钠(SAS)未能完全中和80%和50%(体积比)新鲜人血清的杀菌活性。此外,SAS未能抑制已用0.01 M镁离子加0.01 M乙二醇双(β-氨基乙基醚)-N,N-四乙酸螯合的80%(体积比)新鲜人血清中补体激活的替代途径。然而,250至1000微克/毫升的SAS可有效中和20%(体积比)新鲜人血清的杀菌活性。因此,SAS(250至1000微克/毫升)仅应用于已在合适肉汤培养基中至少稀释五倍(小于或等于20%[体积比])的血样。