淀粉硫酸钠对人血清杀菌活性的中和作用研究。
Studies on neutralization of human serum bactericidal activity by sodium amylosulfate.
作者信息
Traub W H
出版信息
J Clin Microbiol. 1977 Aug;6(2):128-31. doi: 10.1128/jcm.6.2.128-131.1977.
Abstract
The synthetic anticoagulant sodium amylosulfate (SAS) at concentrations of 125 to 2,000 microgram/ml failed to completely neutralize the bactericidal activity of 80 and 50% (by volume) fresh human serum. Furthermore, SAS failed to inhibit the alternative pathway of complement activation in 80% (by volume) fresh human serum that had been chelated with 0.01 M magnesium ions plus 0.01 M ethyleneglycol-bis(beta-aminoethylether)-N,N-tetraacetic acid. However, SAS at 250 to 1,000 microgram/ml effectively neutralized the bactericidal activity of 20% (by volume) fresh human serum. Therefore, SAS (at 250 to 1,000 microgram/ml) should be used only in blood samples that have been diluted at least fivefold (less than or equal to 20% [by volume]) in suitable broth media.
摘要
浓度为125至2000微克/毫升的合成抗凝剂淀粉硫酸酯钠（SAS）未能完全中和80%和50%（体积比）新鲜人血清的杀菌活性。此外，SAS未能抑制已用0.01 M镁离子加0.01 M乙二醇双（β-氨基乙基醚）-N，N-四乙酸螯合的80%（体积比）新鲜人血清中补体激活的替代途径。然而，250至1000微克/毫升的SAS可有效中和20%（体积比）新鲜人血清的杀菌活性。因此，SAS（250至1000微克/毫升）仅应用于已在合适肉汤培养基中至少稀释五倍（小于或等于20%[体积比]）的血样。
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