Peng Fang, Yang Hong, Wu Qin, Liu Jie, Shi Jingzhen
Department of Physiology, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(7):1017-22.
To compare sub-acute toxic effects of cinnabar and Wansheng Huafeng Dan with mercury chloride and methyl-mercury.
Healthy SD rats were orally administered with Wansheng Huafeng Dan (0.42 g x kg(-1)), cinnabar (0.15 g x kg(-1)), HgS (0.15 g x kg(-1)), HgCl2 (0.02 g x kg(-1)), MeHg (0.001 g x kg(-1)) and saline for 21 days under observed and their weights were monitored. After the final administration, they were decapitated and their blood, liver, kidney and brain tissues were collected for calculating hepatic and renal indexes and detecting the contents of serum glutamic pyruvic transaminase, urea nitrogen and creatinine and the mercury accumulation in liver, kidney and brain tissues. Besides, relative expressions of liver metallothionein-1 (MT-1) and cytochrome P450 gene subtypes (Cyp1a1, Cyp2b1, Cyp2e1, Cyp3a2, Cyp4a10) mRNA.
HgCl2 caused obvious weight lose in rats. Mercury contents in liver and kidney were markedly increased by HgCl2 and MeHg, and MeHg markedly increased mercury contents of brain either, but these advent effects were not notable in Wansheng Huafeng Dan and cinnabar groups. However, blood biochemistry and histopathology did not show significant changes in all groups. The expression of rat hepatic MT-1 mRNA was remarkably induced by both HgCl2 and MeHg. The expression of hepatic Cyp3a2 was increased by Wansheng Huafeng Dan and cinnabar, while the expression of Cyp2e1 was inhibited by HgCl2 and MeHg.
The administration of Wansheng Huafeng Dan with equivalent dose for three weeks shows a much low sub-acute toxicity than HgCl2 and MeHg in rats.
比较朱砂和万胜化风丹与氯化汞和甲基汞的亚急性毒性作用。
将健康SD大鼠分别灌胃给予万胜化风丹（0.42 g·kg⁻¹）、朱砂（0.15 g·kg⁻¹）、硫化汞（0.15 g·kg⁻¹）、氯化汞（0.02 g·kg⁻¹）、甲基汞（0.001 g·kg⁻¹）和生理盐水，连续给药21天，观察大鼠体重变化。末次给药后，断头取血，收集肝脏、肾脏和脑组织，计算肝、肾指数，检测血清谷丙转氨酶、尿素氮和肌酐含量以及肝脏、肾脏和脑组织中的汞蓄积量。此外，检测肝脏金属硫蛋白-1（MT-1）和细胞色素P450基因亚型（Cyp1a1、Cyp2b1、Cyp2e1、Cyp3a2、Cyp4a10）mRNA的相对表达量。
氯化汞导致大鼠体重明显减轻。氯化汞和甲基汞显著增加肝脏和肾脏中的汞含量，甲基汞也显著增加脑组织中的汞含量，但万胜化风丹和朱砂组未出现明显上述效应。然而，所有组的血液生化指标和组织病理学检查均未显示出显著变化。氯化汞和甲基汞均显著诱导大鼠肝脏MT-1 mRNA的表达。万胜化风丹和朱砂增加肝脏Cyp3a2的表达，而氯化汞和甲基汞抑制Cyp2e1的表达。
给予大鼠等效剂量的万胜化风丹连续3周，其亚急性毒性远低于氯化汞和甲基汞。
