Department of Environmental Chemistry, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona, Spain.
J Chromatogr A. 2012 Aug 24;1252:115-29. doi: 10.1016/j.chroma.2012.06.078. Epub 2012 Jul 1.
The work describes the development and validation of an analytical method for simultaneous determination of 58 pharmaceuticals and 19 metabolites and transformation products in environmental waters. The method is fully automated and consists of a direct injection of a small volume (several mL) of water sample to an on-line system composed of TurboFlow™ chromatography for the extraction and clean-up followed by liquid chromatography-electrospray-tandem mass spectrometry (TFC-LC-ESI-MS/MS). The feasibility and limitations of the technique in the analysis of environmental and wastewaters is discussed. The main advantages include high throughput, minimum sample manipulation, low error introduction, high selectivity, sensibility and reliability. The method provided a tool for the determination of a high number of active metabolites and transformation products and was successfully applied in the analysis of samples from the river Ebro basin.
该工作描述了一种分析方法的开发和验证，用于同时测定环境水中的 58 种药物和 19 种代谢物和转化产物。该方法完全自动化，包括将小体积（数毫升）水样直接注入由 TurboFlow™色谱法组成的在线系统，用于提取和净化，然后进行液相色谱-电喷雾串联质谱法（TFC-LC-ESI-MS/MS）。讨论了该技术在环境和废水中分析的可行性和局限性。主要优点包括高通量、最小的样品处理、低误差引入、高选择性、灵敏度和可靠性。该方法为测定大量活性代谢物和转化产物提供了一种工具，并成功应用于分析埃布罗河流域的样品。
