Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khoram Abad, Iran.
J Diabetes Complications. 2012 Nov-Dec;26(6):476-82. doi: 10.1016/j.jdiacomp.2012.06.004. Epub 2012 Jul 12.
Coenzyme Q10 is a natural antioxidant and scavenging free radicals. In the present study, we examined antioxidative activities of coenzyme Q10 and possible protective effect of coenzyme Q10 on in vivo and in vitro lipid peroxidation, antioxidant enzymes activity and glomerulosclerosis in alloxan-induced type 1 diabetic rats. Thirty Sprague-Dawley male rats were divided into three groups randomly: group 1 as control, group 2 as diabetic untreatment, and group 3 as treatments with coenzyme Q10 by 15 mg/kg i.p. daily, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After 8 weeks, animals were anaesthetized, liver and kidney were then removed immediately and used fresh or kept frozen until their lipid peroxidation analysis. Blood samples were also collected before killing to measure the lipid peroxidation and antioxidant enzymes activity. Kidney paraffin sections were prepared and stained by periodic acid-Schiff method. Glomerular volume and leukocyte infiltration were estimated by stereological rules and glomerular sclerosis was studied semi-quantitatively. Coenzyme Q10 significantly inhibited leukocyte infiltration, glomerulosclerosis and the levels of malondialdehyde (MDA) serum and kidney content in treated group compared with the diabetic untreated group. Coenzyme Q10 significantly inhibited LDL oxidation in vitro. Coenzyme Q10 significantly increased the serum levels of glutathione (GSH) and serum activity of catalase (CAT) and superoxide dismutase (SOD) in treated group compared with the diabetic untreated group. Coenzyme Q10 alleviates leukocyte infiltration and glomerulosclerosis and exerts beneficial effects on the lipid peroxidation and antioxidant enzymes activity in alloxan-induced type 1 diabetic rats.
辅酶 Q10 是一种天然抗氧化剂，可以清除自由基。在本研究中，我们研究了辅酶 Q10 的抗氧化活性以及辅酶 Q10 对体外和体内脂质过氧化、抗氧化酶活性和丙烯醛诱导的 1 型糖尿病大鼠肾小球硬化的可能保护作用。30 只雄性 Sprague-Dawley 大鼠随机分为三组：第 1 组为对照组，第 2 组为糖尿病未治疗组，第 3 组为每天 15mg/kg 腹腔注射辅酶 Q10 治疗组。第 2 和第 3 组大鼠通过皮下注射丙烯醛诱导糖尿病。8 周后，动物麻醉，立即取出肝脏和肾脏，用于新鲜或冷冻直至进行脂质过氧化分析。在处死前采集血液样本以测量脂质过氧化和抗氧化酶活性。制备肾脏石蜡切片，并用过碘酸-Schiff 法染色。用体视学规则估计肾小球体积和白细胞浸润，并半定量研究肾小球硬化。与糖尿病未治疗组相比，辅酶 Q10 治疗组显著抑制白细胞浸润、肾小球硬化和血清及肾脏丙二醛（MDA）含量。辅酶 Q10 显著抑制 LDL 的体外氧化。与糖尿病未治疗组相比，辅酶 Q10 治疗组血清谷胱甘肽（GSH）水平和血清过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性显著升高。辅酶 Q10 减轻白细胞浸润和肾小球硬化，并对丙烯醛诱导的 1 型糖尿病大鼠的脂质过氧化和抗氧化酶活性产生有益影响。
