Nasri Maryam, Mahdavifard Sina, Babaeenezhad Esmaeel, Adibhesami Glavizh, Nouryazdan Negar, Veiskarami Saeid, Rahimi Monfared Sobhan, Birjandi Mehdi, Ahmadvand Hassan
Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Khorramabad, Iran.
Growth and Development Research Center, Tehran University of Medical Science, Tehran, Iran.
Iran J Basic Med Sci. 2020 Jun;23(6):714-723. doi: 10.22038/ijbms.2020.38553.9148.
The present study sought to evaluate the beneficial effects of histidine (His) on oxidative stress, tumor necrosis factor alpha (TNF-α), renal histological alterations and anti-oxidant enzymes gene expressions in type 2 diabetic rats.
Streptozotocin/nicotinamide (STZ/NA) induced diabetic rats were used as an animal model of type 2 diabetes. One group of rats received daily His (1000 mg/l) in drinking water for 8 weeks, whereas other groups (control and untreated diabetic groups) received only water. Different parameters such as glucose, insulin, insulin resistance, lipid profile, cardiac risk ratios, renal functional markers, and oxidative stress were determined in all groups. Moreover, renal histological alterations, mRNA expressions of anti-oxidant enzymes, and TNF-α were evaluated in the rats.
His exhibited a protective effect on glucose, insulin, insulin resistance, lipid profile, cardiac risk ratios, renal functional markers, oxidative stress, and TNF-α. Furthermore, His restored the renal histological alterations and normalized the augmented mRNA expressions of renal anti-oxidant enzymes (glutathione peroxidase (GPX) and Cu-Zn superoxide dismutase (Cu-Zn SOD)) and TNF-α.
His could ameliorate diabetes complications related to oxidative stress, inflammation, dyslipidemia, hyperglycemia, insulin resistance, and nephropathy. Hence, the use of this amino acid is recommended for diabetic patients in order to reduce diabetes complications.
本研究旨在评估组氨酸（His）对2型糖尿病大鼠氧化应激、肿瘤坏死因子α（TNF-α）、肾脏组织学改变及抗氧化酶基因表达的有益作用。
将链脲佐菌素/烟酰胺（STZ/NA）诱导的糖尿病大鼠作为2型糖尿病动物模型。一组大鼠每天饮用含His（1000 mg/l）的水，持续8周，而其他组（对照组和未治疗的糖尿病组）只饮水。测定所有组的不同参数，如血糖、胰岛素、胰岛素抵抗、血脂谱、心脏风险比、肾功能标志物和氧化应激。此外，评估大鼠的肾脏组织学改变、抗氧化酶的mRNA表达和TNF-α。
His对血糖、胰岛素、胰岛素抵抗、血脂谱、心脏风险比、肾功能标志物、氧化应激和TNF-α具有保护作用。此外，His恢复了肾脏组织学改变，并使肾脏抗氧化酶（谷胱甘肽过氧化物酶（GPX）和铜锌超氧化物歧化酶（Cu-Zn SOD））及TNF-α的mRNA表达增加恢复正常。
His可改善与氧化应激、炎症、血脂异常、高血糖、胰岛素抵抗和肾病相关的糖尿病并发症。因此，建议糖尿病患者使用这种氨基酸以减少糖尿病并发症。
