Moar M H, Rosén A, Klein G
J Immunol Methods. 1979;30(3):219-29. doi: 10.1016/0022-1759(79)90096-6.
The present paper describes the use of [125I]Protein A, isolated from Staphylococcus aureus, in detecting antigen-antibody complexes by autoradiography on single cells. the method is relatively quick, reproducible, potentially more sensitive than immunofluorescence, and should be useful in combination with conventional radioimmuno-assays. We have used it to detect the cellular expression of IgM, kappa, lambda, and beta 2-microglobulin, as well as the expression of Epstein-Barr Virus (EBV)-associated antigens expressed in human lymphoblastoid cell lines.
本文描述了从金黄色葡萄球菌中分离出的[125I]蛋白A在通过单细胞放射自显影检测抗原-抗体复合物中的应用。该方法相对快速、可重复，可能比免疫荧光更灵敏，并且与传统放射免疫测定法结合使用应该会很有用。我们已用它来检测IgM、κ、λ和β2-微球蛋白的细胞表达，以及在人淋巴母细胞系中表达的爱泼斯坦-巴尔病毒（EBV）相关抗原的表达。
