Sumaya C V, Ench Y, Pope R M
J Infect Dis. 1982 Oct;146(4):518-23. doi: 10.1093/infdis/146.4.518.
The determination of levels of IgM antibody to Epstein-Barr virus (EBV) capsid antigen was improved by absorbing unfractionated test serum with Staphylococcus aureus cells containing protein A. Nonspecific background fluorescence was significantly decreased, thus facilitating the reading of slides and the detection of specific fluorescent reactions in serum with low levels of this IgM antibody. Moreover, the inclusion of the absorption step eliminated false-positive reactions caused by the presence of rheumatoid factor in serum and shortened the time needed for the incubation of test serum with the EBV-infected cell smear. This improved antibody test should facilitate the laboratory diagnosis of acute EBV infections, including unaccompanied by a heterophil antibody response.
通过用含有蛋白A的金黄色葡萄球菌细胞吸收未分级的测试血清，改进了针对爱泼斯坦-巴尔病毒（EBV）衣壳抗原的IgM抗体水平的测定。非特异性背景荧光显著降低，从而便于读取玻片并检测血清中低水平这种IgM抗体时的特异性荧光反应。此外，加入吸收步骤消除了血清中类风湿因子导致的假阳性反应，并缩短了测试血清与EBV感染细胞涂片孵育所需的时间。这种改进的抗体检测方法应有助于急性EBV感染的实验室诊断，包括无嗜异性抗体反应的情况。
