Gerdes J C, McNally I, Hileman L, Burks J S
J Immunol Methods. 1982 Oct 29;54(2):191-202. doi: 10.1016/0022-1759(82)90060-6.
Autoradiographic methods can be used as an alternative to indirect immunofluorescence to detect viral antigen expression or the presence of IgG in tissue sections. Iodinated protein A isolated from Staphylococcus aureus detects an influx of IgG into the central nervous system of mice inoculated with the coronavirus SD. Antispecies antibody that has been iodinated detects coronavirus antigen expression for 24 days post-inoculation while it is only detectable for 10 days by immunofluorescence. A direct comparison of indirect fluorescence and autoradiographic methods indicates that the autoradiographic techniques are considerably more sensitive. This increased sensitivity is sufficient to permit the detection of viral antigen in formalin fixed paraffin embedded tissue sections.
放射自显影方法可作为间接免疫荧光的替代方法，用于检测组织切片中的病毒抗原表达或IgG的存在。从金黄色葡萄球菌中分离出的碘化蛋白A可检测到接种冠状病毒SD的小鼠中枢神经系统中IgG的流入。碘化的抗种属抗体在接种后24天可检测到冠状病毒抗原表达，而通过免疫荧光仅在10天内可检测到。间接荧光法和放射自显影方法的直接比较表明，放射自显影技术的灵敏度要高得多。这种提高的灵敏度足以在福尔马林固定石蜡包埋的组织切片中检测到病毒抗原。
