Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
Exp Parasitol. 2012 Oct;132(2):216-21. doi: 10.1016/j.exppara.2012.07.003. Epub 2012 Jul 14.
A total of 597 faecal samples were collected from western grey kangaroos (Macropus fuliginosus), Euros (M. robustus), red kangaroos (M. rufus) in Western Australia and Eastern Grey Kangaroos (M. giganteus) from Victoria and screened for the presence of Eimeria by PCR at the 18S ribosomal RNA (rRNA) locus. The overall prevalence was 24.3% (145/597). At the 18S rRNA locus, sequences were obtained for 25 of the 145 positives. Phylogenetic analysis indicated that all the macropod-derived Eimeria species grouped in a separate marsupial clade that included Eimeria trichosuri from brushtail possums. At least 6 different clades were identified within the marsupial isolates and many of the genotypes identified are likely to be valid species, however morphological and biological data need to be collected to match sequences to previously characterized Eimeria species or identify if they are new species.
从西澳大利亚的西部灰袋鼠（Macropus fuliginosus）、欧洲袋鼠（M. robustus）、红袋鼠（M. rufus）以及维多利亚州的东部灰袋鼠（M. giganteus）中收集了总共 597 份粪便样本，通过聚合酶链反应（PCR）在 18S 核糖体 RNA（rRNA）基因座上对这些样本进行了艾美耳球虫的存在情况筛查。总体流行率为 24.3%（145/597）。在 18S rRNA 基因座上，从 145 个阳性样本中获得了 25 个序列。系统发育分析表明，所有源自有袋动物的艾美耳球虫物种都聚集在一个单独的有袋动物分支中，其中包括来自帚尾袋貂的艾美耳短圆艾美耳球虫。在有袋动物分离株中至少鉴定出了 6 个不同的分支，其中许多鉴定出的基因型可能是有效的物种，但是需要收集形态学和生物学数据来将序列与先前表征的艾美耳球虫物种相匹配，或者确定它们是否是新物种。
