Indonesian Research Center for Veterinary Science, Bogor, 16114, Indonesia.
Department of Parasitology, Veterinary Medicine, Gadjah Mada University, Yogyakarta, 55281, Indonesia.
Parasitol Res. 2020 Apr;119(4):1271-1279. doi: 10.1007/s00436-020-06618-2. Epub 2020 Feb 18.
To date, more than 50 Eimeria spp. have been isolated from marsupials of the family Macropodidae. Although 18 species of Eimeria have been previously detected from multiple animal species belonging to the genus Macropus of the family, limited genetic analyses of the parasites are available, and their pathogenicity remains unclear. Here, we report the isolation of Eimeria spp. from a zoo specimen of red-necked wallaby (Macropodidae; Macropus rufogriseus). Specifically, two distinct types of Eimeria oocysts were recovered, one from the feces before treatment with an anthelmintic and the second from the intestinal contents after death of the animal. The oocysts obtained from the two sources were morphologically identified as E. hestermani and E. prionotemni, respectively. We successfully determined partial gene sequences from the two isolates, including segments of the 18S rRNA genes, and for the first time have used phylogenetic analyses of these sequences to assign the species to distinct clades. In combination with further genetic data, these results are expected to help elucidate the pathogenicity and host ranges of Eimeria spp. within the respective family and genus.
迄今为止，已从有袋目（Macropodidae）的袋貂科（Macropodidae）中分离出超过 50 种艾美耳球虫。虽然先前已从属于袋貂属（Macropus）的多个动物物种中检测到 18 种艾美耳球虫，但对寄生虫的遗传分析有限，其致病性仍不清楚。在这里，我们报告了从动物园的红颈袋鼠（Macropodidae；Macropus rufogriseus）标本中分离出的艾美耳球虫。具体而言，从驱虫前的粪便和动物死后的肠内容物中分别回收了两种不同类型的艾美耳球虫卵囊。从这两个来源获得的卵囊在形态上分别被鉴定为艾美耳球虫（Eimeria hestermani）和艾美耳球虫（E. prionotemni）。我们成功地从两种分离物中确定了部分基因序列，包括 18S rRNA 基因的片段，并首次使用这些序列的系统发育分析将这些物种分配到不同的进化枝。结合进一步的遗传数据，这些结果有望帮助阐明各自科和属内艾美耳球虫的致病性和宿主范围。
