School of Life Sciences, Hunan University of Science and Technology, Taoyuan Rd., Xiangtan, 411201, Hunan, China.
Planta. 2012 Nov;236(5):1485-98. doi: 10.1007/s00425-012-1698-7. Epub 2012 Jul 14.
WRKY transcription factors are crucial regulatory components of plant responses to pathogen infection. In the present study, we report isolation and functional characterization of the pathogen-responsive rice WRKY30 gene, whose transcripts accumulate rapidly in response to salicylic acid (SA) and jasmonic acid (JA) treatment. Overexpression of WRKY30 in rice enhanced resistance to rice sheath blight fungus Rhizoctonia solani and blast fungus Magnaporthe grisea. The enhanced resistance in the transgenic lines overexpressing WRKY30 was associated with activated expression of JA synthesis-related genes LOX, AOS2 and pathogenesis-related (PR)3 and PR10, and increased endogenous JA accumulation under the challenge of fungal pathogens. WRKY30 was nuclear-localized and had transcriptional activation ability in yeast cells, supporting that it functions as a transcription factor. Together, our findings indicate that JA plays a crucial role in the WRKY30-mediated defense responses to fungal pathogens, and that the rice WRKY30 seems promising as an important candidate gene to improve disease resistance in rice.
WRKY 转录因子是植物对病原体感染反应的关键调节成分。在本研究中，我们报告了对病原体响应的水稻 WRKY30 基因的分离和功能表征，其转录物在响应水杨酸（SA）和茉莉酸（JA）处理时迅速积累。在水稻中超表达 WRKY30 增强了对水稻纹枯病菌和稻瘟病菌的抗性。在过表达 WRKY30 的转基因系中增强的抗性与 JA 合成相关基因 LOX、AOS2 和病程相关（PR）3 和 PR10 的激活表达以及真菌病原体挑战下内源 JA 的积累增加有关。WRKY30 定位于核内，并在酵母细胞中具有转录激活能力，支持其作为转录因子发挥作用。总之，我们的研究结果表明，JA 在 WRKY30 介导的对真菌病原体的防御反应中起关键作用，并且水稻 WRKY30 似乎是提高水稻抗病性的一个有前途的重要候选基因。
