Asia-Pacific Centre for Animal Health, Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
Science. 2012 Jul 13;337(6091):188. doi: 10.1126/science.1217134.
Recombination between herpesviruses has been seen in vitro and in vivo under experimental conditions. This has raised safety concerns about using attenuated herpesvirus vaccines in human and veterinary medicine and adds to other known concerns associated with their use, including reversion to virulence and disease arising from recurrent reactivation of lifelong chronic infection. We used high-throughput sequencing to investigate relationships between emergent field strains and vaccine strains of infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1). We show that independent recombination events between distinct attenuated vaccine strains resulted in virulent recombinant viruses that became the dominant strains responsible for widespread disease in Australian commercial poultry flocks. These findings highlight the risks of using multiple different attenuated herpesvirus vaccines, or vectors, in the same populations.
疱疹病毒在实验条件下的体外和体内发生重组，这引起了人们对在人和兽医医学中使用减毒疱疹病毒疫苗的安全性的担忧，并增加了与使用这些疫苗相关的其他已知问题，包括毒力回复和由终身慢性感染的反复再激活引起的疾病。我们使用高通量测序来研究传染性喉气管炎病毒（ILTV，禽疱疹病毒 1）的新出现的田间株和疫苗株之间的关系。我们表明，不同减毒疫苗株之间的独立重组事件导致了毒力重组病毒的出现，这些病毒成为了导致澳大利亚商业家禽群中广泛疾病的主要毒株。这些发现强调了在同一人群中使用多种不同减毒疱疹病毒疫苗或载体的风险。
