Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100085, PR China.
Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1285-1290. doi: 10.1099/ijs.0.042101-0. Epub 2012 Jul 13.
A gram-negative, rod-shaped, aerobic, motile bacterium, strain Y1243-1(T), was isolated from an ice core drilled from Yuzhufeng Glacier, Tibetan Plateau, China. Cells had polar flagella. The novel strain shared 94.7-97.6 % 16S rRNA gene sequence similarity with the type strains of species of the genus Massilia. The novel isolate is thus classified in the genus Massilia. The major fatty acids of strain Y1243-1(T) were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) (43.98 %), C16 : 0 (27.86 %), C10 : 0 3-OH (7.10 %), C18 : 0 (6.95 %) and C18 : 1ω7c (5.01 %). The predominant isoprenoid quinone was Q-8. The DNA G+C content of strain Y1243-1(T) was 65.7 mol% (Tm). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. A number of phenotypic characteristics distinguished the novel isolate from the type strains of recognized Massilia species. Furthermore, in DNA-DNA hybridization tests, strain Y1243-1(T) shared 45 % relatedness with its closest phylogenetic relative, Massilia consociata CCUG 58010(T). From the genotypic and phenotypic data, it is evident that strain Y1243-1(T) represents a novel species of the genus Massilia, for which the name Massilia yuzhufengensis sp. nov. is proposed. The type strain is Y1243-1(T) ( = KACC 16569(T) = CGMCC 1.12041(T)).
一株革兰氏阴性、杆状、需氧、能动的细菌，菌株 Y1243-1(T)，从中国青藏高原玉珠峰冰川的冰芯中分离出来。细胞具有极性鞭毛。新型菌株与马西利亚属种的模式菌株的 16S rRNA 基因序列相似性为 94.7-97.6%。因此，新型分离株被分类为马西利亚属。菌株 Y1243-1(T)的主要脂肪酸为总和特征 3（C16:1ω7c 和/或 iso-C15:0 2-OH）（43.98%）、C16:0（27.86%）、C10:0 3-OH（7.10%）、C18:0（6.95%）和 C18:1ω7c（5.01%）。主要的异戊烯醌是 Q-8。菌株 Y1243-1(T)的 DNA G+C 含量为 65.7mol%（Tm）。主要的极性脂是磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。新型分离株与公认的马西利亚种的模式菌株在许多表型特征上有所不同。此外，在 DNA-DNA 杂交试验中，菌株 Y1243-1(T)与最接近的亲缘关系菌株 Massilia consociata CCUG 58010(T)的相关性为 45%。从基因型和表型数据来看，菌株 Y1243-1(T)代表了马西利亚属的一个新种，建议将其命名为 Massilia yuzhufengensis sp. nov.。该模式菌株为 Y1243-1(T)（=KACC 16569(T)=CGMCC 1.12041(T)）。
