Industrial Bio-materials Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
Energy Materials and Process, BK 21, Hanyang University, Seoul 133-791, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1304-1310. doi: 10.1099/ijs.0.033134-0. Epub 2012 Jul 13.
Two strains of Gram-staining-negative, rod-shaped bacteria that were motile by gliding, N7d-4(T) and B4a-b5, were isolated during a study of culturable bacteria in soil cultivated with potatoes. These isolates grew at 15-37 °C and at pH 6.5-7.0. The major cellular fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), anteiso-C15 : 0, iso-C15 : 0, iso-C17 : 0 3-OH and iso-C17 : 1ω9c. The major polar lipids were phosphatidyl-N-methylethanolamine and phosphatidylethanolamine. The strains contained d-18 : 0 and d-19 : 0 sphingosines. The DNA G+C contents of strains N7d-4(T) and B4a-b5 were 48.5 and 46.9 mol% (HPLC), respectively. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains N7d-4(T) and B4a-b5 were affiliated with Pedobacter species in the family Sphingobacteriaceae. Strains N7d-4(T) and B4a-b5 shared 99.9 % sequence similarity, and the most closely related Pedobacter type strains were Pedobacter composti TR6-06(T) (96.5 and 96.7 % sequence similarity, respectively), P. oryzae N7(T) (95.4 and 95.6 %) and P. caeni LMG 22862(T) (94.0 and 94.4 %). Phenotypic data and phylogenetic inference clearly distinguished the two isolates from other Pedobacter species. Based on these data, the isolates are considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter luteus sp. nov. is proposed. The type strain is N7d-4(T) ( = KCTC 22699(T) = DSM 22385(T)).
两株革兰氏阴性、杆状、可滑行运动的细菌 N7d-4(T) 和 B4a-b5 是在研究土豆栽培土壤中可培养细菌时分离得到的。这些分离物在 15-37°C 和 pH6.5-7.0 下生长。主要细胞脂肪酸为总和特征 3（异-C15：0 2-OH 和/或 C16：1ω7c）、anteiso-C15：0、异-C15：0、异-C17：0 3-OH 和异-C17：1ω9c。主要极性脂为磷脂-N-甲乙醇胺和磷脂乙醇胺。菌株含有 d-18：0 和 d-19：0 神经酰胺。菌株 N7d-4(T) 和 B4a-b5 的 DNA G+C 含量分别为 48.5 和 46.9 mol%（HPLC）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 N7d-4(T) 和 B4a-b5 属于鞘脂杆菌科 Pedobacter 属。菌株 N7d-4(T) 和 B4a-b5 之间的序列相似度为 99.9%，与最接近的 Pedobacter 型菌株分别为 Pedobacter composti TR6-06(T)（96.5%和 96.7%序列相似度）、P. oryzae N7(T)（95.4%和 95.6%序列相似度）和 P. caeni LMG 22862(T)（94.0%和 94.4%序列相似度）。表型数据和系统发育推断清楚地区分了这两个分离物与其他 Pedobacter 种。基于这些数据，这些分离物被认为代表了 Pedobacter 属的一个新种，建议将其命名为 Pedobacter luteus sp. nov.。模式菌株为 N7d-4(T)（=KCTC 22699(T)=DSM 22385(T)）。
