Pedobacter paludis sp. nov., isolated from wetland soil.

A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated YX, was isolated from wetland soil. Compared to strain YX, Pedobacter yonginense HMD1002 had the highest 16S rRNA gene sequence identity (97.8%), and the remaining strains had the identities below 97%. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain YX grouped with P. yonginense HMD1002. The values of DNA-DNA hybridization and genomic orthologous average nucleotide identity (orthoANI) between strain YX and Pedobacter yonginense KCTC 22721 were 58.5% and 82.0%, respectively. The genome size of strain YX was 5.0 Mb, comprising 4369 predicted genes with a DNA G+C content of 37.3 mol %. Strain YX had menaquinone-7 as the only respiratory quinone and polar lipids of phosphatidylethanolamine, sphingolipid, aminolipid and three unidentified lipids. The predominant cellular fatty acids (> 10%) of strain YX were summed feature 3 (iso-C 2-OH and/or Cω7c), iso-C and iso-C 3-OH. Strain YX could be distinguished from the other Pedobacter members based on the data of phylogenetic distance, DNA-DNA hybridization, genomic orthoANI, RecA MLSA, core-protein comparison, and hydrolyses of L-arginine, utilization of D/L-lactate, L-alanine, 5-ketonic gluconate and glycogen. Therefore, strain YX represents a novel species of the genus Pedobacter, for which the name Pedobacter paludis sp. nov. is proposed. The type strain is YX (= KCTC 62520 = CCTCC AB 2018029).