Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.
Poult Sci. 2012 Aug;91(8):1819-24. doi: 10.3382/ps.2011-02129.
A series of experiments were conducted to study the basal amounts of vitamin D-1α-hydroxylase and vitamin D-24-hydroxylase mRNA amounts in different organs and the effect of immune stimulation on 1α- and 24-hydroxylase mRNA amounts in chickens. At day of hatch, kidneys had an approximately 66-fold higher amount of 1α-hydroxylase and 550-fold higher amount of 24-hydroxylase mRNA, thigh and breast muscles had an approximately 20-fold higher amount of 1α-hydroxylase mRNA, and the thymus had an approximately 41-fold higher amount of 24-hydroxylase mRNA than the liver. An in vivo LPS injection did not alter the amount of 1α-hydroxylase mRNA in the breast muscle (P=0.60) or in the kidneys (P=0.39). An in vivo LPS injection decreased (P=0.01) the amount of 24-hydroxylase mRNA in the breast muscle at 3 d post-LPS injection. An in vivo LPS injection increased (P=0.01) the amount of 24-hydroxylase mRNA in the kidneys at 2, 3, and 6 d post-LPS injection. An in vitro stimulation altered amounts of 1α- (P=0.01) and 24-hydroxylase (P=0.04) mRNA in CD4+ cells. In conclusion, the distribution of 1α- and 24-hydroxylase mRNA amounts was similar to mammals, and an immune stimulation altered the amounts of 1α- and 24-hydroxylase mRNA in chickens.
进行了一系列实验，以研究不同器官中维生素 D-1α-羟化酶和维生素 D-24-羟化酶 mRNA 含量的基础水平，以及免疫刺激对鸡中 1α-和 24-羟化酶 mRNA 含量的影响。在孵化日，肾脏中 1α-羟化酶的 mRNA 含量约高 66 倍，24-羟化酶的 mRNA 含量约高 550 倍，大腿和胸肌中 1α-羟化酶的 mRNA 含量约高 20 倍，而胸腺中 24-羟化酶的 mRNA 含量比肝脏高约 41 倍。体内 LPS 注射不会改变胸肌（P=0.60）或肾脏中 1α-羟化酶 mRNA 的含量（P=0.39）。体内 LPS 注射会降低胸肌中 24-羟化酶 mRNA 的含量（P=0.01），在 LPS 注射后 3 天。体内 LPS 注射会增加肾脏中 24-羟化酶 mRNA 的含量（P=0.01），在 LPS 注射后 2、3 和 6 天。体外刺激改变了 CD4+细胞中 1α-（P=0.01）和 24-羟化酶（P=0.04）mRNA 的含量。总之，1α-和 24-羟化酶 mRNA 含量的分布与哺乳动物相似，免疫刺激会改变鸡中 1α-和 24-羟化酶 mRNA 的含量。
