Department of Animal Science, College of Agriculture, University of Zabol, Zabol, Iran.
Department of Animal Science, College of Agriculture, Lorestan University, Lorestan, Iran.
Trop Anim Health Prod. 2022 Aug 10;54(5):254. doi: 10.1007/s11250-022-03257-w.
The present study was conducted to evaluate aflatoxin B (AFB) detoxification effects of some medicinal plants under both in vitro and in vivo. The in vitro experiment was performed with 25 treatments in 5 replications. The samples of medicinal plants were incubated with AFB for 72 h, and the toxin residual in the supernatant was determined. The highest aflatoxin elimination was found to be related to peppermint (81%). Thereafter, in vivo experiment was conducted to investigate the effects of peppermint dried leaves, essential oil, and menthol on liver, bone, and meat, as well as the performance of growing Japanese quail fed diet contaminated with AFB. A total of 640 7-day-old Japanese quails were assigned using a completely randomized design as 2 × 4 factorial arrangement with two levels of AFB (including 0 and 2.5 mg/kg diet) and four treatments (including no additive; peppermint powder, 20 g/kg; peppermint essential oil, 800 mg/kg; and menthol powder, 400 mg/kg). Those birds fed AFB-contaminated diet with no additives showed the worst liver health status by considering super oxide dismutase (P = 0.0399), glutathione peroxidase (P = 0.0139), alanine aminotransferase (P < 0.0001), and aspartate aminotransferase levels (P = 0.0512). However, the supplementation of AFB contaminated diet with additives improved their liver health status. Menthol receiving birds showed the highest tibia strength, while the birds fed with AFB-contaminated diet with no additives had the weakest bone strength (P < 0.0001). A significant increase was also observed in malondialdehyde level of meat by dietary inclusion of AFB, which was well-repressed by the dietary supplementation of peppermint essential oil and menthol (P = 0.0075). Body weight gain dramatically decreased by adding AFB to the diet, which was recovered with the dietary supplementation of additives (P = 0.0585). According to the results of the current study, peppermint and its derivatives can be used to suppress aflatoxin effects on the liver, bone, and meat quality and to improve the performance of Japanese quails.
本研究旨在评估一些药用植物在体外和体内条件下对黄曲霉毒素 B (AFB) 的解毒效果。体外实验采用 25 种处理方法，重复 5 次。将药用植物样本与 AFB 孵育 72 小时，然后测定上清液中残留的毒素。结果发现，对黄曲霉毒素的最高消除率与薄荷有关（81%）。此后，进行了体内实验，以研究薄荷干叶、精油和薄荷醇对肝脏、骨骼和肌肉的影响，以及在饲料中添加 AFB 对生长日本鹌鹑性能的影响。将 640 只 7 日龄日本鹌鹑完全随机设计，分为 2×4 因子设计，两个 AFB 水平（包括饲料中 0 和 2.5mg/kg）和四个处理组（包括无添加剂；20g/kg 薄荷粉；800mg/kg 薄荷精油；400mg/kg 薄荷脑）。考虑到超氧化物歧化酶（P=0.0399）、谷胱甘肽过氧化物酶（P=0.0139）、丙氨酸氨基转移酶（P<0.0001）和天冬氨酸氨基转移酶水平（P=0.0512），添加 AFB 污染饲料且未添加任何添加剂的鸟类肝脏健康状况最差。然而，添加 AFB 污染饲料的添加剂可改善其肝脏健康状况。接受薄荷醇的鸟类表现出最强的胫骨强度，而添加 AFB 污染饲料且未添加任何添加剂的鸟类的骨骼强度最弱（P<0.0001）。膳食中添加 AFB 也显著提高了肉中的丙二醛水平，而膳食中添加薄荷精油和薄荷醇则能很好地抑制其升高（P=0.0075）。在饲料中添加 AFB 会导致体重显著下降，而添加添加剂可恢复体重（P=0.0585）。根据本研究的结果，薄荷及其衍生物可用于抑制黄曲霉毒素对肝脏、骨骼和肌肉品质的影响，并提高日本鹌鹑的性能。
