Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Poult Sci. 2012 Aug;91(8):1846-53. doi: 10.3382/ps.2011-01830.
Two experiments were performed to screen bacilli isolated from quails for their aflatoxin removal potential and to assess the efficiency of their amelioration of experimental aflatoxicosis. Nonhemolytic bacilli were selected for in vitro aflatoxin B1 (AFB1) removal and conventional probiotic tests. The isolate with the highest scores was selected for assessment in field experiments and was identified as Berevibacillus laterosporus (Bl). In the second experiment, 125 male Japanese quails (21 d old) were divided into 5 groups with 5 replications to compare the toxin removal efficiency of Bl with that of a commercial toxin binder, improved Millbond-TX (IMTX). The experimental groups were as follows: Control (without any feed additive or AFB1); AFB1 (2.5 mg/kg); AFB1+Bl (2.5 mg/kg+10(8) cfu/mL); AFB1+IMTX (2.5 mg/kg+2.5 g/kg); and Bl (10(8) cfu/mL). The greatest BW gain and slaughter and carcass weights were found in the Bl group and the lowest values were observed in the AFB1 group (P<0.05). Feeding AFB1 alone to the chicks resulted in a significant decrease in serum albumin, total protein, and glucose and cholesterol levels but a significant increase in serum uric acid, urea, creatinin and phosphorus (P<0.05). Treatment of birds on AFB1 with Bl restored these to their original levels (P<0.05). AFB1+Bl-fed birds had serum aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and alkaline phosphatase enzyme activity similar to control birds (P<0.05). Antibody titer against Newcastle disease virus was found to be lowest in the AFB1 group but highest in the Bl group (P<0.05). Antibody production against sheep red blood cells was lower in the AFB1 group compared with the AFB1+Bl group (P<0.05). Berevibacillus laterosporus supplementation of the AFB1 diet restored the skin response to 2,4-dinitro 1-chlorobenzene to levels comparable with control birds (P<0.05). It can be concluded that selected indigenous Bl is a promising probiotic with AFB1 removal potential.
进行了两项实验，以筛选具有脱除黄曲霉毒素潜力的鹌鹑源杆菌，并评估其改善实验性黄曲霉毒素中毒的效果。选择非溶血杆菌进行体外黄曲霉毒素 B1（AFB1）去除和常规益生菌测试。选择得分最高的分离株进行田间实验评估，并鉴定为迟缓芽孢杆菌（Bl）。在第二项实验中，将 125 只雄性日本鹌鹑（21 日龄）分为 5 组，每组 5 个重复，以比较 Bl 和商业毒素结合剂改良 Millbond-TX（IMTX）的毒素去除效率。实验组如下：对照组（无任何饲料添加剂或 AFB1）；AFB1（2.5 mg/kg）；AFB1+Bl（2.5 mg/kg+10^8 cfu/mL）；AFB1+IMTX（2.5 mg/kg+2.5 g/kg）；和 Bl（10^8 cfu/mL）。Bl 组的 BW 增重和屠宰及胴体重量最大，AFB1 组的最低（P<0.05）。单独给雏鸡喂食 AFB1 会导致血清白蛋白、总蛋白和葡萄糖以及胆固醇水平显著降低，但血清尿酸、尿素、肌酐和磷水平显著升高（P<0.05）。用 Bl 处理喂食 AFB1 的鸟类可将这些指标恢复到原来的水平（P<0.05）。AFB1+Bl 喂养的鸟类的血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶、乳酸脱氢酶和碱性磷酸酶的活性与对照组相似（P<0.05）。AFB1 组的新城疫病毒抗体滴度最低，而 Bl 组的最高（P<0.05）。与 AFB1+Bl 组相比，AFB1 组的绵羊红细胞抗体产生较低（P<0.05）。用 AFB1 饲料补充迟缓芽孢杆菌可将皮肤对 2,4-二硝基-1-氯苯的反应恢复到与对照组相似的水平（P<0.05）。可以得出结论，选择的本地 Bl 是一种具有脱除 AFB1 潜力的有前途的益生菌。
