United Graduate School of Agricultural Science, Kagoshima University, Korimoto, Kagoshima, Japan.
J Agric Food Chem. 2012 Aug 15;60(32):7973-80. doi: 10.1021/jf301848s. Epub 2012 Jul 31.
Citrus peels are important sources of various pleasant aroma compounds and valuable bioactive substances. To investigate differences in the composition and content of Shiikuwasha (Citrus depressa Hayata) peels from different cultivation lines, the composition of volatile aroma components, flavanones, and polymethoxylated flavones (PMFs) in four Shiikuwasha cultivation lines was examined. The composition of volatile aroma components in cold-pressed extracts of Shiikuwasha peels was analyzed using gas chromatography-flame ionization detection and gas chromatography-mass spectrophotometry. The extracts contained mainly monoterpene hydrocarbons (93.40-97.25%), including limonene (46.52-68.26%) and γ-terpinene (21.48-30.52%). Differences in the composition of volatile aroma compounds in the Shiikuwasha cultivation lines were revealed using principal component analysis. Additionally, the composition of flavanones and PMFs was determined using high-performance liquid chromatography methods. Neohesperidin (96.58%) was the predominant flavanone in 'Izumi kugani' peel, while the other peels had high hesperidin contents (89.26-98.66%). Moreover, the PMFs of Shiikuwasha peels were composed of nobiletin (56.74-64.77%) and tangeretin (23.17-34.70%).
柑橘皮是各种令人愉悦的香气化合物和有价值的生物活性物质的重要来源。为了研究不同栽培品种的使君子皮在组成和含量上的差异，本研究考察了四个使君子栽培品种的冷榨提取物中挥发性香气成分、类黄酮和多甲氧基黄酮（PMFs）的组成。采用气相色谱-火焰离子化检测和气相色谱-质谱联用技术分析了使君子皮冷榨提取物中挥发性香气成分的组成。提取物主要含有单萜烃（93.40-97.25%），包括柠檬烯（46.52-68.26%）和γ-萜品烯（21.48-30.52%）。利用主成分分析揭示了使君子栽培品种中挥发性香气化合物组成的差异。此外，采用高效液相色谱法测定了类黄酮和 PMFs 的组成。在‘Izumi kugani’皮中，新橙皮苷（96.58%）是主要的类黄酮，而其他皮则含有高含量的柚皮苷（89.26-98.66%）。此外，使君子皮的 PMFs 由诺米林（56.74-64.77%）和川陈皮素（23.17-34.70%）组成。
