Coca-Prados M, Hsu M T
J Virol. 1979 Jul;31(1):199-208. doi: 10.1128/JVI.31.1.199-208.1979.
The simian virus 40 virion assembly process was studied with pulse-labeling kinetics of virion proteins, CsCl gradient analysis, electron microscopy, and low-salt gel electrophoresis. The results obtained are consistent with the model of gradual addition and organization of capsid proteins around simian virus 40 chromatin. Empty virions, as observed in the CsCl gradient by previous workers, were found to be the dissociation product of immature virus. Histone H1 was found in simian virus 40 chromatin and virion assembly intermediates but not in the mature virion banding at 1.34 g/ml in the CsCl gradient.
利用病毒体蛋白的脉冲标记动力学、氯化铯梯度分析、电子显微镜和低盐凝胶电泳对猴病毒40病毒体装配过程进行了研究。所得结果与衣壳蛋白围绕猴病毒40染色质逐步添加和组织的模型一致。如先前研究人员在氯化铯梯度中观察到的那样，空病毒体被发现是未成熟病毒的解离产物。在猴病毒40染色质和病毒体装配中间体中发现了组蛋白H1，但在氯化铯梯度中密度为1.34 g/ml的成熟病毒体条带中未发现。
