Universität Regensburg, Biochemie-Zentrum Regensburg (BZR), Lehrstuhl Biochemie III, Regensburg, Germany.
EMBO J. 2012 Aug 15;31(16):3480-93. doi: 10.1038/emboj.2012.185. Epub 2012 Jul 17.
Several DNA cis-elements and trans-acting factors were described to be involved in transcription termination and to release the elongating RNA polymerases from their templates. Different models for the molecular mechanism of transcription termination have been suggested for eukaryotic RNA polymerase I (Pol I) from results of in vitro and in vivo experiments. To analyse the molecular requirements for yeast RNA Pol I termination, an in vivo approach was used in which efficient termination resulted in growth inhibition. This led to the identification of a Myb-like protein, Ydr026c, as bona fide termination factor, now designated Nsi1 (NTS1 silencing protein 1), since it was very recently described as silencing factor of ribosomal DNA. Possible Nsi1 functions in regard to the mechanism of transcription termination are discussed.
有几个 DNA 顺式元件和反式作用因子被描述为参与转录终止，并将延伸的 RNA 聚合酶从其模板上释放出来。不同的模型已经被提出用于真核 RNA 聚合酶 I（Pol I）的分子机制的研究，这些模型是基于体外和体内实验的结果。为了分析酵母 RNA Pol I 终止的分子要求，采用了一种体内方法，其中有效的终止导致生长抑制。这导致了一种 Myb 样蛋白 Ydr026c 的鉴定，它是真正的终止因子，现在被命名为 Nsi1（NTS1 沉默蛋白 1），因为它最近被描述为核糖体 DNA 的沉默因子。讨论了 Nsi1 在转录终止机制中的可能功能。
