Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA.
1] Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA [2] Department of Epidemiology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA [3] Department of Pediatrics, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
J Expo Sci Environ Epidemiol. 2014 Jan-Feb;24(1):3-8. doi: 10.1038/jes.2012.76. Epub 2012 Jul 18.
Airborne mouse allergen is a risk factor for respiratory diseases. Conventional assessment techniques provide mass-based exposure estimates that may not capture completely the inhalation risk of airborne allergen particles. In contrast to mass-based estimates, the halogen immunoassay (HIA) combines immunoblotting and microscopy to directly assess allergen-containing particles. We evaluated the HIA for the assessment of airborne mouse allergen and compared the results to the enzyme linked immunosorbent assay (ELISA). Particulate matter (PM)(10) and PM(2.5) samples (30 min, 4 l/m) were collected in a mouse facility before, during, and after disturbance of soiled bedding. Concentrations of Mus m 1-positive particles (haloed particles (HPs)) and intensities of the haloes were determined with the HIA. Although HPs/m(3) were positively correlated with mass concentration (statistically significant only with Mus m 1 concentration on PM(10)), replicates of mass concentration showed higher variability than HPs/m(3). After disturbance, most of the HPs were in the PM(2.5) fraction. Mean haloes intensities were similar before, during, and after disturbance. The HIA was able to measure allergen-containing particles with less variability than the ELISA, detected the shift of HPs to smaller particles after disturbance, and may suggests similar halo intensity by particles detected during and after disturbance. Our findings suggest that the HIA can be used to assess indoor concentrations of mouse allergen particles and their morphological characteristics.
空气中的鼠过敏原是呼吸道疾病的一个风险因素。传统的评估技术提供的是基于质量的暴露估计，可能无法完全捕捉到空气中过敏原颗粒的吸入风险。与基于质量的估计相比，卤化物免疫测定 (HIA) 结合免疫印迹和显微镜直接评估含过敏原的颗粒。我们评估了 HIA 用于评估空气中的鼠过敏原，并将结果与酶联免疫吸附测定 (ELISA) 进行了比较。在污染垫料被扰动之前、期间和之后，在一个鼠设施中收集了 PM(10) 和 PM(2.5) （30 分钟，4 l/m）的颗粒物样本。使用 HIA 确定了 Mus m 1 阳性颗粒（晕轮颗粒 (HPs)) 的浓度和晕轮的强度。虽然 HPs/m(3) 与质量浓度呈正相关（仅在 PM(10)上与 Mus m 1 浓度具有统计学意义），但质量浓度的重复显示出更高的变异性。扰动后，大多数 HPs 处于 PM(2.5) 部分。在扰动之前、期间和之后，平均晕轮强度相似。与 ELISA 相比，HIA 能够以较小的变异性测量含过敏原的颗粒，检测到 HPs 在扰动后向较小颗粒的转移，并且可能表明在扰动期间和之后检测到的颗粒具有相似的晕轮强度。我们的研究结果表明，HIA 可用于评估室内鼠过敏原颗粒的浓度及其形态特征。
