Ohman J L, Hagberg K, MacDonald M R, Jones R R, Paigen B J, Kacergis J B
Allergy Department, New England Medical Center, Boston.
J Allergy Clin Immunol. 1994 Nov;94(5):810-7. doi: 10.1016/0091-6749(94)90147-3.
Occupational allergy to mice is a major cause of disability among workers in mouse breeding and research facilities. Efforts to prevent and treat allergy require a detailed knowledge of exposure levels to allergen.
This study was designed to quantitate the level of major mouse allergen (Mus m I) in central room air and immediate breathing zones under a variety of working conditions.
An Andersen sampler (Groseby Andersen, Spirotech Div., Atlanta, Ga.) was used to collect allergen in each room. A Gillian Personal sampler (Gillian Instrument Corp., West Caldwell, N.J.) collected particles in the worker breathing zone. ELISA was used to quantitate the concentration of Mus m I collected on the two collection devices.
Total Mus m I recovered from Andersen samplers ranged from 0.2 to 1.5 ng/m3 in rooms without mice and 0.5 to 15.1 ng/m3 in rooms with mice. Allergen recovered from the zone of worker activity ranged from 1.2 to 2.7 ng/m3 in rooms without mice and from 16.6 to 563.0 ng/m3 in rooms with mice. Direct mouse contact was associated with the highest levels of exposure to Mus m I. Analysis revealed the bulk of allergen to be in mid-particle size ranges (3.3 to 10 microns) for mouse-containing rooms and in small particle size range (0.43 to 3.3 microns) for non-mouse-containing rooms, suggesting that small particles were carried along corridors from rooms with mice into non-mouse-containing rooms. Ventilation characteristics of rooms and mouse population density were evaluated with a "mouse loading" index (number of mice per cubic meter of ventilated air per hour). Mouse loading correlated strongly with small particles (< 3.3 microns) in ambient air.
Mus m I is widely distributed within mouse breeding facilities. Direct worker contact with mice seems to be the major factor in high level exposure.
职业性小鼠过敏是小鼠饲养和研究设施中工作人员致残的主要原因。预防和治疗过敏的努力需要详细了解过敏原的暴露水平。
本研究旨在定量各种工作条件下中央房间空气和直接呼吸区域中主要小鼠过敏原（Mus m I）的水平。
使用安德森采样器（Groseby Andersen，Spirotech Div.，亚特兰大，佐治亚州）在每个房间收集过敏原。吉利安个人采样器（Gillian Instrument Corp.，西考德威尔，新泽西州）在工作人员呼吸区域收集颗粒物。采用酶联免疫吸附测定法（ELISA）定量两个收集装置上收集的Mus m I浓度。
在没有小鼠的房间里，从安德森采样器回收的总Mus m I范围为0.2至1.5纳克/立方米，在有小鼠的房间里为0.5至15.1纳克/立方米。在没有小鼠的房间里，从工作人员活动区域回收的过敏原范围为1.2至2.7纳克/立方米，在有小鼠的房间里为16.6至563.0纳克/立方米。直接接触小鼠与最高水平的Mus m I暴露相关。分析表明，对于有小鼠的房间，大部分过敏原存在于中等粒径范围（3.3至10微米），对于没有小鼠的房间，存在于小粒径范围（0.43至3.3微米），这表明小颗粒从有小鼠的房间沿着走廊进入没有小鼠的房间。用“小鼠负荷”指数（每立方米通风空气每小时的小鼠数量）评估房间的通风特性和小鼠种群密度。小鼠负荷与环境空气中的小颗粒（<3.3微米）密切相关。
Mus m I在小鼠饲养设施中广泛分布。工作人员直接接触小鼠似乎是高暴露水平的主要因素。
