Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-713, Republic of Korea.
J Microbiol. 2011 Feb;49(1):29-34. doi: 10.1007/s12275-011-0315-y. Epub 2011 Mar 3.
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)).
一株新型的柴油和正十六烷降解细菌菌株，命名为 DR1(T)，从韩国德寿的稻田中分离得到。菌株 DR1(T)细胞呈革兰氏阴性、需氧短杆菌，在 20-37°C 下生长，最适温度为 30°C，最适 pH 值为 6-8。有趣的是，菌株 DR1(T) 利用菌毛高度运动（泳动和滚动运动），并产生 N-酰基高丝氨酸内酯作为群体感应信号。鉴定出的主要呼吸醌为泛醌-9（Q-9），DNA G+C 含量为 41.4mol%。基于 16S rRNA 基因序列的比较系统发育分析将该菌株置于与 A. calcoaceticus、A. haemolyticus、A. baumannii、A. baylyi 和 A. beijerinckii 等种属的一个分支中，与这些种属的 16S rRNA 基因序列相似性分别为 98.2%、97.4%、97.2%、97.1%和 97.0%。菌株 DR1(T) 与其他不动杆菌属种属间的 DNA-DNA 杂交值均小于 20%。生理和分类特征以及 DNA-DNA 杂交数据支持将菌株 DR1(T) 鉴定为不动杆菌属的一个新种，命名为 Acinetobacter oleivorans sp. nov.。模式菌株为 DR1(T)（=KCTC 23045(T)=JCM 16667(T)）。
