Bulgarian Academy of Sciences, The Stephan Angeloff Institute of Microbiology, Acad. G. Bonchev Str., Bl. 26, 1113 Sofia, Bulgaria.
World J Microbiol Biotechnol. 2012 May;28(5):2069-76. doi: 10.1007/s11274-012-1009-2. Epub 2012 Feb 7.
This study demonstrated the potential of microbial isolates from Antarctic soils to produce hydrolytic enzymes by using specific substrates. The results revealed potential of the strains to produce a broad spectrum of hydrolytic enzymes. Strain A-1 isolated from soil samples in Casey Station, Wilkes Land, was identified as Nocardioides sp. on the basis of morphological, biochemical, physiological observations and also chemotaxonomy analysis. Enzymatic and antimicrobial activities of the cell-free supernatants were explored after growth of strain A-1 in mineral salts medium supplemented with different carbon sources. It was found that the carbon sources favored the production of a broad spectrum of enzymes as well as compounds with antimicrobial activity against Gram-positive and Gram-negative bacteria, especially Staphylococcus aureus and Xanthomonas oryzae. Preliminary analysis showed that the compounds with antimicrobial activity produced by the strain A-1 are mainly glycolipids and/or lipopeptides depending on the used carbon source. The results revealed a great potential of the Antarctic Nocardioides sp. strain A-1 for biotechnological, biopharmaceutical and biocontrol applications as a source of industrially important enzymes and antimicrobial/antifungal compounds.
本研究利用特定的底物证明了来自南极土壤的微生物分离物产生水解酶的潜力。结果显示了这些菌株产生广谱水解酶的潜力。根据形态学、生物化学、生理观察以及化学分类学分析，从威尔克斯地凯西站土壤样本中分离出的菌株 A-1 被鉴定为诺卡氏菌属。在补充不同碳源的无机盐培养基中培养菌株 A-1 后，探索了其无细胞上清液的酶活性和抗菌活性。结果发现，碳源有利于产生广谱酶以及对革兰氏阳性和革兰氏阴性细菌（特别是金黄色葡萄球菌和稻黄单胞菌）具有抗菌活性的化合物。初步分析表明，菌株 A-1 产生的具有抗菌活性的化合物主要是糖脂和/或脂肽，具体取决于所使用的碳源。结果表明，南极诺卡氏菌属菌株 A-1 具有巨大的生物技术、生物制药和生物防治应用潜力，可作为工业上重要的酶和抗菌/抗真菌化合物的来源。
