Instituto de Cerámica de Galicia, Universidad Santiago de Compostela, Avda Mestre Mateo S/N, 15706 Santiago de Compostela, Spain.
J Mater Sci Mater Med. 2012 Oct;23(10):2471-82. doi: 10.1007/s10856-012-4717-0. Epub 2012 Jul 18.
This work describes the synthesis of chlorapatite single crystals using the molten salt method with CaCl(2) as a flux. By manipulating the processing conditions (amount of flux, firing time and temperature, and cooling rates) it is possible to manipulate the crystal morphology from microscopic fibres to large crystals (up to few millimetre long and ~100 μm thick). The crystal roughness can be controlled to achieve very flat surfaces by changing the melt composition "in situ" at high temperature. The Young modulus and hardness of the crystals are 110 ± 15 and 6.6 ± 1.5 GPa respectively as measured by nanoindentation. Crystal dissolution in Hanks solution starts around the defects. Several in vitro assays were performed; ClAp crystals with different size and shape are biocompatible. Cell apoptosis was very low at 5, 10, and 15 days (Caspase-3) for all the samples. Proliferation (MTT) showed to be influenced by surface roughness and size of the crystals.
这项工作描述了使用熔融盐法合成氯磷灰石单晶，其中以 CaCl(2) 作为助熔剂。通过控制处理条件（助熔剂的用量、煅烧时间和温度以及冷却速率），可以控制晶体形态从微观纤维到较大晶体（长达几毫米，厚度约 100μm）。通过在高温下“原位”改变熔体成分，可以控制晶体粗糙度，从而获得非常平坦的表面。通过纳米压痕法测量，晶体的杨氏模量和硬度分别为 110±15 和 6.6±1.5GPa。晶体在 Hanks 溶液中的溶解始于缺陷周围。进行了多项体外检测；不同大小和形状的 ClAp 晶体具有生物相容性。所有样品在第 5、10 和 15 天（Caspase-3）的细胞凋亡率都非常低。增殖（MTT）显示受晶体表面粗糙度和尺寸的影响。
