Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Curr Microbiol. 2012 Nov;65(5):516-23. doi: 10.1007/s00284-012-0190-8. Epub 2012 Jul 18.
Wolbachia and Cardinium are maternally inherited intracellular bacteria that can manipulate the reproduction of their arthropod hosts, such as by inducing cytoplasmic incompatibility (CI). Although the reproductive alteration induced by Wolbachia or Cardinium have been well investigated, the effects of these two endosymbionts co-infecting the same host are poorly understood. We found that Tetranychus piercei McGregor is naturally infected with Wolbachia and Cardinium. We performed all possible crossing combinations using naturally infected and cured strains, and the results show that Wolbachia induced a weak level of CI, while Cardinium-infected and doubly infected males caused severe CI. Wolbachia and Cardinium could not rescue CI each other; however, Wolbachia boosted the expression of Cardinium-induced CI. Quantitative PCR results demonstrated that CI was associated with the infection density of Wolbachia and Cardinium.
沃尔巴克氏体和卡他霉素是两种母系遗传的胞内细菌，能够操纵其节肢动物宿主的繁殖，例如通过诱导细胞质不亲和（CI）。尽管沃尔巴克氏体或卡他霉素诱导的生殖改变已经得到了很好的研究，但这两种内共生体同时感染同一宿主的影响还知之甚少。我们发现，麦格雷戈氏棉叶螨自然感染沃尔巴克氏体和卡他霉素。我们使用自然感染和治愈的品系进行了所有可能的杂交组合，结果表明沃尔巴克氏体诱导了弱水平的 CI，而感染卡他霉素的和双重感染的雄性导致严重的 CI。沃尔巴克氏体和卡他霉素不能相互挽救 CI；然而，沃尔巴克氏体增强了卡他霉素诱导的 CI 的表达。定量 PCR 结果表明，CI 与沃尔巴克氏体和卡他霉素的感染密度有关。
