Merlin Bruna Laís, Moraes Gilberto J, Cônsoli Fernando L
Department of Entomology and Acarology, College of Agriculture Luiz de Queiroz, University of São Paulo, Piracicaba, SP, Brazil.
CNPq, Federal District, Brazil.
Microb Ecol. 2023 May;85(4):1590-1607. doi: 10.1007/s00248-022-02032-6. Epub 2022 May 11.
Microbiota has diverse roles in the life cycles of their hosts, affecting their growth, development, behavior, and reproduction. Changes in physiological conditions of the host can also impact the assemblage of host-associated microorganisms. However, little is known of the effects of host plant-prey-predatory mite interactions on mite microbiota. We compared the microbial communities of eggs and adult females of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), and of adult females of the predatory mite Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) on four different host plants (cotton, maize, pinto bean, and tomato) by metabarcoding sequencing of the V3-V4 region of the 16S ribosomal RNA gene (16S rRNA), using the Illumina MiSeq platform. Only the egg microbiota of T. urticae was affected by the host plant. The microbiota of the predatory mite N. californicus was very different from that of its prey, and the predator microbiota was unaffected by the different host plant-prey systems tested. Only the microbiota of the eggs of T. urticae carried Serratia as a high fidelity-biomarker, but their low abundance in T. urticae adult females suggests that the association between Serratia and T. urticae is accidental. Biomarker bacteria were also detected in the microbiota of adult females of T. urticae and N. californicus, with different biomarkers in each host plant species. The microbiota associated with eggs and adult females of T. urticae and adult females of N. californicus differed in their functional potential contributions to the host mite.
微生物群在其宿主的生命周期中具有多种作用，影响宿主的生长、发育、行为和繁殖。宿主生理条件的变化也会影响宿主相关微生物的组合。然而，关于宿主植物-猎物-捕食螨相互作用对螨类微生物群的影响知之甚少。我们通过对16S核糖体RNA基因（16S rRNA）的V3-V4区域进行元条形码测序，使用Illumina MiSeq平台，比较了二斑叶螨（Tetranychus urticae Koch，蜱螨目：叶螨科）的卵和成年雌螨以及加州新小绥螨（Neoseiulus californicus，McGregor，蜱螨目：植绥螨科）的成年雌螨在四种不同宿主植物（棉花、玉米、菜豆和番茄）上的微生物群落。只有二斑叶螨的卵微生物群受到宿主植物的影响。捕食螨加州新小绥螨的微生物群与其猎物的微生物群非常不同，并且捕食者的微生物群不受所测试的不同宿主植物-猎物系统的影响。只有二斑叶螨卵的微生物群携带沙雷氏菌作为高保真生物标志物，但它们在二斑叶螨成年雌螨中的丰度较低，这表明沙雷氏菌与二斑叶螨之间的关联是偶然的。在二斑叶螨和加州新小绥螨成年雌螨的微生物群中也检测到了生物标志物细菌，每种宿主植物物种中的生物标志物都不同。与二斑叶螨的卵和成年雌螨以及加州新小绥螨成年雌螨相关的微生物群在对宿主螨的功能潜在贡献方面存在差异。
