Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine Science and Engineering, University of Science and Technology, Tianjin, 300457, China.
World J Microbiol Biotechnol. 2012 Jan;28(1):31-7. doi: 10.1007/s11274-011-0789-0. Epub 2011 May 22.
To isolate a salt tolerant hydrogen-producing bacterium, we used the sludge from the intertidal zone of a bathing beach in Tianjin as inoculum to enrich hydrogen-producing bacteria. The sludge was treated by heat-shock pretreatment with three different temperature (80, 100 and 121°C) respectively. A hydrogen-producing bacterium was isolated from the sludge pretreated at 80°C by sandwich plate technique and identified using microscopic examination and 16S rDNA gene sequence analysis. The isolated bacterium was named as Bacillus sp. B2. The present study examined the hydrogen-producing ability of Bacillus sp. B2. The strain was able to produce hydrogen over a wide range of initial pH from 5.0 to 10.0, with an optimum at pH 7.0. The level of hydrogen production was also affected by the salt concentration. Strain B2 has unique capability to adapt high salt concentration. It could produce hydrogen at the salt concentration from 4 to 60‰. The maximum of hydrogen-producing yield of strain B2 was 1.65 ± 0.04 mol H(2)/mol glucose (mean ± SE) at an initial pH value of 7.0 in marine culture conditions. Hydrogen production under fresh culture conditions reached a higher level than that in marine ones. As a result, it is likely that Bacillus sp. B2 could be applied to biohydrogen production using both marine and fresh organic waste.
为了分离耐盐产氢细菌，我们以天津海滨浴场潮间带底泥为接种物，通过热处理（80、100 和 121°C）对产氢细菌进行了富集。采用夹层平板法从 80°C 热处理的底泥中分离出一株产氢细菌，并通过显微镜观察和 16S rDNA 基因序列分析对其进行了鉴定。该分离出的细菌被命名为芽孢杆菌 B2。本研究考察了芽孢杆菌 B2 的产氢能力。该菌株在初始 pH 值为 5.0 到 10.0 的较宽范围内都具有产氢能力，最适 pH 值为 7.0。产氢水平也受到盐浓度的影响。B2 菌株具有适应高盐浓度的独特能力。它可以在盐浓度为 4 到 60‰的条件下产氢。B2 菌株在初始 pH 值为 7.0 的海洋培养条件下的最大产氢产率为 1.65±0.04 mol H2/mol 葡萄糖（平均值±SE）。在新鲜培养条件下的产氢水平高于海洋培养条件。因此，芽孢杆菌 B2 可能适用于利用海洋和新鲜有机废物生产生物氢气。
