Zhu Zhi, Song Yanling, Li Cong, Zou Yuan, Zhu Ling, An Yuan, Yang Chaoyong James
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University , Xiamen 361005, P. R. China.
Anal Chem. 2014 Jun 17;86(12):5881-8. doi: 10.1021/ac501423g. Epub 2014 Jun 6.
A novel method, monoclonal surface display SELEX (MSD-SELEX), has been designed for simple, rapid, efficient, and cost-effective enrichment and identification of aptamers from a library of monoclonal DNA-displaying beads produced via highly parallel single-molecule emulsion PCR. The approach was successfully applied for the identification of high-affinity aptamers that bind specifically to different types of targets, including cancer biomarker protein EpCAM and small toxin molecule aflatoxin B1. Compared to the conventional sequencing-chemical synthesis-screening work flow, MSD-SELEX avoids large-scale DNA sequencing, expensive and time-consuming DNA synthesis, and labor-intensive screening of large populations of candidates, thus offering a new approach for simple, rapid, efficient, and cost-effective aptamer identification for a wide variety of applications.
一种名为单克隆表面展示SELEX（MSD-SELEX）的新方法，已被设计用于从通过高度平行的单分子乳液PCR产生的单克隆DNA展示珠文库中，简单、快速、高效且经济高效地富集和鉴定适体。该方法已成功应用于鉴定与不同类型靶标特异性结合的高亲和力适体，包括癌症生物标志物蛋白EpCAM和小毒素分子黄曲霉毒素B1。与传统的测序-化学合成-筛选工作流程相比，MSD-SELEX避免了大规模DNA测序、昂贵且耗时的DNA合成以及对大量候选物进行劳动密集型筛选，从而为各种应用提供了一种简单、快速、高效且经济高效的适体鉴定新方法。
