Ohta A, Mayo M C, Kramer N, Lands W E
Department of Biological Chemistry, University of Illinois, Chicago 60680.
Lipids. 1990 Nov;25(11):742-7. doi: 10.1007/BF02544044.
A rapid and convenient procedure for the quantitative determination of the fatty acid composition of plasma lipids is described. Human plasma was applied directly to the preadsorbent zones of thin-layer silica gel plates with added antioxidant, internal standards and carriers. The thin-layer chromatography (TLC) plates were partially developed with methanol followed by chloroform/methanol (1:1, v/v), and then they were fully developed in hexane/diethyl ether/acetic acid (80:20:1, v/v/v) to separate the major classes of lipids. Silica gel from regions containing the separated lipids was scraped into screw-capped tubes and treated with boron trifluoride-methanol prior to gas chromatography. The method of direct application to TLC plates gave yields and compositions of fatty acids very similar to the method of applying extracted plasma lipids. This relatively simple method is suitable for analyzing the fatty acids in plasma lipids from a 50 microliter finger-tip blood samples from an individual, and it may be useful in wide-scale screening of different individuals to estimate the relative amounts of ingested polyunsaturated fatty acids.
本文描述了一种快速便捷的定量测定血浆脂质脂肪酸组成的方法。将人体血浆直接应用于添加了抗氧化剂、内标物和载体的薄层硅胶板的预吸附区。薄层色谱（TLC）板先用甲醇部分展开，然后用氯仿/甲醇（1:1，v/v）展开，接着在己烷/乙醚/乙酸（80:20:1，v/v/v）中完全展开以分离主要脂质类别。将含有分离脂质区域的硅胶刮入带螺旋盖的试管中，并在气相色谱分析前用三氟化硼 - 甲醇处理。直接应用于TLC板的方法所得到的脂肪酸产率和组成与应用提取的血浆脂质的方法非常相似。这种相对简单的方法适用于分析来自个体的50微升指尖血样中血浆脂质的脂肪酸，并且可能有助于大规模筛查不同个体以估计摄入的多不饱和脂肪酸的相对量。
