Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
World J Microbiol Biotechnol. 2012 Feb;28(2):453-61. doi: 10.1007/s11274-011-0836-x. Epub 2011 Jul 9.
A chemically defined medium called KGm medium was used to isolate from a sample of sea water a bacterial strain, MW3A, capable of using N-3-oxohexanoyl-L: -homoserine lactone as the sole carbon source. MW3A was clustered closely to Pseudomonas aeruginosa by 16S ribosomal DNA sequence analysis. It degraded both N-acylhomoserine lactones (AHLs) with a 3-oxo group substitution and, less preferably, AHLs with unsubstituted groups at C3 position in the acyl side chain, as determined by Rapid Resolution Liquid Chromatography. Its quiP and pvdQ homologue gene sequences showed high similarities to those of known acylases. Spent supernatant of MW3A harvested at 8-h post inoculation was shown to contain long-chain AHLs when assayed with the biosensor Escherichia coli [pSB1075], and specifically N-dodecanoyl-L: -homoserine lactone and N-3-oxotetradecanoyl-L: -homoserine lactone by high resolution mass spectrometry. Hence, we report here a novel marine P. aeruginosa strain MW3A possessing both quorum-quenching and quorum-sensing properties.
使用一种称为 KGm 培养基的化学成分确定的培养基，从海水样本中分离出一株能够将 N-3-氧代己酰-L: -高丝氨酸内酯用作唯一碳源的细菌菌株 MW3A。MW3A 通过 16S 核糖体 DNA 序列分析与铜绿假单胞菌聚类密切。通过快速分辨液相色谱法测定，它可以降解具有 3-酮取代基的 N-酰基高丝氨酸内酯（AHLs），并且在酰基侧链的 C3 位置取代的 AHLs 降解能力较弱。其 quiP 和 pvdQ 同源基因序列与已知的酰基酶具有高度相似性。当用生物传感器大肠杆菌 [pSB1075] 检测时，MW3A 在接种后 8 小时收获的废上清液中显示出长链 AHLs 的存在，特别是 N-十二烷酰基-L: -高丝氨酸内酯和 N-3-氧代十四烷酰基-L: -高丝氨酸内酯通过高分辨率质谱法。因此，我们在这里报道了一种新型海洋铜绿假单胞菌 MW3A，它具有群体感应和群体淬灭特性。
