Wong Cheng-Siang, Yin Wai-Fong, Sam Choon-Kook, Koh Chong-Lek, Chan Kok-Gan
Division of Genetics and Molecular Biology, University of Malaya, Kuala Lumpur, Malaysia.
New Microbiol. 2012 Jan;35(1):43-51. Epub 2012 Jan 11.
Most Proteobacteria produce N-acylhomoserine lactones for bacterial cell-to-cell communication, a process called quorum sensing. Interference of quorum sensing, commonly known as quorum quenching, represents an important way to control quorum sensing. This work reports the isolation of quorum quenching bacterium strain 2WS8 from Malaysia tropical wetland water (2°11'8"N, 102°15'2"E, in 2007) by using a modified version of a previously reported KG medium. Strain 2WS8 was isolated based on its ability to utilize N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) as the sole source of energy. This bacterium clustered closely to Pseudomonas aeruginosa PAO1. Strain 2SW8 possesses both quiP and pvdQ homologue acylase genes. Rapid Resolution Liquid Chromatography analysis confirmed that strain 2SW8 preferentially degraded N-acylhomoserine lactones with 3-oxo group substitution but not those with unsubstituted groups at C3 position in the acyl side chain. Strain 2SW8 also showed 2-heptyl-3-hydroxy-4-quinolone production.
大多数变形菌会产生N-酰基高丝氨酸内酯用于细菌细胞间通讯，这一过程称为群体感应。干扰群体感应，通常称为群体猝灭，是控制群体感应的一种重要方式。本研究报告了通过使用先前报道的KG培养基的改良版本，从马来西亚热带湿地水（2007年，北纬2°11'8"，东经102°15'2"）中分离出群体猝灭菌株2WS8。菌株2WS8是根据其利用N-（3-氧代己酰基）-L-高丝氨酸内酯（3-氧代-C6-HSL）作为唯一能量来源的能力而分离出来的。该细菌与铜绿假单胞菌PAO1紧密聚类。菌株2SW8同时拥有quiP和pvdQ同源酰基转移酶基因。快速分离液相色谱分析证实，菌株2SW8优先降解在酰基侧链C3位置带有3-氧代基团取代的N-酰基高丝氨酸内酯，而不是那些在C3位置没有取代基团的N-酰基高丝氨酸内酯。菌株2SW8还显示出2-庚基-3-羟基-4-喹诺酮的产生。
