从饮用水中分离出的中生根瘤菌F7的群体感应活性。

Quorum sensing activity of Mesorhizobium sp. F7 isolated from potable water.

作者信息

Yong Pei-Ling, Chan Kok-Gan

机构信息

Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

出版信息

ScientificWorldJournal. 2014;2014:874764. doi: 10.1155/2014/874764. Epub 2014 Aug 6.

DOI:10.1155/2014/874764

PMID:25177734

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4142172/

Abstract

We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely, N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) and N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions.

摘要

我们从饮用水中分离出一株细菌菌株（F7）。通过16S rDNA基因系统发育分析，该菌株被鉴定为中生根瘤菌属，并利用AHL生物传感器筛选其N-酰基高丝氨酸内酯（AHL）的产生情况。使用高分辨率三重四极杆液相色谱质谱联用仪（LC/MS）进一步分析该分离株的AHL图谱，证实其产生了多种AHL，即N-3-氧代辛酰-L-高丝氨酸内酯（3-氧代-C8-HSL）和N-3-氧代癸酰-L-高丝氨酸内酯（3-氧代-C10-HSL）。这些发现将为研究这些AHL在植物-微生物相互作用中的功能开辟前景。