Veterinary Pathology, Department of Veterinary Medicine, Federal University of Lavras, Lavras, MG, Brazil.
Histol Histopathol. 2012 Sep;27(9):1227-30. doi: 10.14670/HH-27.1227.
The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer than that with direct immunofluorescence. The protocol provided a provisory histopathological rabies diagnosis in approximately three and half hours and the immunohistochemical diagnosis was available after six hours. The protocol achieved 100% correlation with direct immunofluorescence and is a promising method, particularly in situations in which only material in formalin is available for diagnosis or when the refrigeration or transportation of biological material is difficult.
本研究描述了一种微波处理方案在牛狂犬病的快速组织病理学和免疫组织化学诊断中的应用。免疫组织化学已被用于福尔马林固定组织的狂犬病诊断，结果令人满意，尽管诊断所需的时间明显长于直接免疫荧光法。该方案大约在三个半小时内提供了临时的组织病理学狂犬病诊断，六小时后即可进行免疫组织化学诊断。该方案与直接免疫荧光法的相关性达到 100%，是一种很有前途的方法，特别是在仅可使用福尔马林固定材料进行诊断的情况下，或者在冷藏或运输生物材料困难的情况下。
