Tomasina Florencia, Carabio Claudio, Celano Laura, Thomson Leonor
Enzymology Laboratory, Facultad de Ciencias, Universidad de la República, Igua 4225, Montevideo 11400, Uruguay.
Biochem Mol Biol Educ. 2012 Jul;40(4):266-70. doi: 10.1002/bmb.20617. Epub 2012 Jun 23.
This exercise is intended to introduce undergraduate biochemistry students to the analysis of antioxidants as a biotechnological tool. In addition, some statistical resources will also be used and discussed. Antioxidants play an important metabolic role, preventing oxidative stress-mediated cell and tissue injury. Knowing the antioxidant content of nutritional components can help make informed decisions about diet design, and increase the commercial value of antioxidant-rich natural products. As a reliable and convenient technique to evaluate the whole spectrum of antioxidants present in biological samples is lacking, the general consensus is to use more than one technique. We have chosen two widely used and inexpensive methods, Trolox-equivalent antioxidant capacity and the ferric reducing antioxidant power assays, to evaluate the antioxidant content of several fruits, and to compare and analyze the correlation between both assays.
本练习旨在向本科生物化学专业学生介绍将抗氧化剂分析作为一种生物技术工具。此外，还将使用和讨论一些统计资源。抗氧化剂发挥着重要的代谢作用，可防止氧化应激介导的细胞和组织损伤。了解营养成分中的抗氧化剂含量有助于在饮食设计方面做出明智决策，并提高富含抗氧化剂的天然产品的商业价值。由于缺乏一种可靠且便捷的技术来评估生物样品中存在的全谱抗氧化剂，目前普遍的共识是使用多种技术。我们选择了两种广泛使用且成本低廉的方法，即Trolox等效抗氧化能力法和铁还原抗氧化能力测定法，来评估几种水果的抗氧化剂含量，并比较和分析这两种测定方法之间的相关性。
