Xiao Jun, Chen Jun-Xing, Zhu Yu-Ping, Zhou Lin-Yu, Shu Qi-An, Chen Ling-Wu
Department of Urology, Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui.
Oncol Lett. 2012 Jul;4(1):113-118. doi: 10.3892/ol.2012.673. Epub 2012 Apr 5.
Mitochondrial tumor suppressor 1 (MTUS1) is a newly identified candidate tumor suppressor gene. Previous studies have demonstrated that the expression status of MTUS1 is altered in several types of tumors. However, its clinical significance for bladder cancer patients remains undetermined. In this study, we detected the expression of MTUS1 mRNA in bladder tumors and paired normal samples obtained from 5 patients using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). A significant downregulation of MTUS1 mRNA expression was observed in the tumor tissues compared with the corresponding normal bladder tissue (P<0.001). We further tested the expression of MTUS1 mRNA in 55 bladder cancer tissues and 10 adjacent normal bladder tissues by quantitative real-time RT-PCR. Correlations between MTUS1 and clinicopathological features and prognosis were investigated by statistical analyses. The results showed that MTUS1 expression was correlated with tumor grade, stage, size and number (P<0.001, P<0.001, P=0.034 and P=0.029, respectively). Patients with low levels of MTUS1 mRNA expression had a poor prognosis compared with those with a high expression (P<0.001). Univariate and multivariate logistic regression prognostic analyses revealed that MTUS1 mRNA was an independent prognostic factor for disease-free survival in bladder cancer (P<0.05). In conclusion, these data suggest that MTUS1 is significant in the progression of bladder cancer and that the status of MTUS1 mRNA expression is a novel prognostic marker for predicting bladder tumor disease-free survival.
线粒体肿瘤抑制因子1（MTUS1）是一个新发现的候选肿瘤抑制基因。先前的研究表明，MTUS1的表达状态在几种类型的肿瘤中发生改变。然而，其对膀胱癌患者的临床意义仍未确定。在本研究中，我们使用半定量逆转录-聚合酶链反应（RT-PCR）检测了5例患者的膀胱肿瘤及配对正常样本中MTUS1 mRNA的表达。与相应的正常膀胱组织相比，肿瘤组织中MTUS1 mRNA表达显著下调（P<0.001）。我们进一步通过定量实时RT-PCR检测了55例膀胱癌组织和10例相邻正常膀胱组织中MTUS1 mRNA的表达。通过统计分析研究了MTUS1与临床病理特征及预后之间的相关性。结果显示，MTUS1表达与肿瘤分级、分期、大小和数量相关（分别为P<0.001、P<0.001、P=0.034和P=0.029）。与高表达患者相比，MTUS1 mRNA表达水平低的患者预后较差（P<0.001）。单因素和多因素逻辑回归预后分析显示，MTUS1 mRNA是膀胱癌无病生存的独立预后因素（P<0.05）。总之，这些数据表明MTUS1在膀胱癌进展中具有重要意义，且MTUS1 mRNA表达状态是预测膀胱肿瘤无病生存的一种新的预后标志物。
