Shi Kebin, Edwards Perry S, Hu Jing, Xu Qian, Wang Yanming, Psaltis Demetri, Liu Zhiwen
Biomed Opt Express. 2012 Jul 1;3(7):1744-9. doi: 10.1364/BOE.3.001744. Epub 2012 Jun 27.
CARS holography captures both the amplitude and the phase of a complex anti-Stokes field, and can perform three-dimensional imaging by digitally focusing onto different depths inside a specimen. The application of CARS holography for bio-imaging is demonstrated. It is shown that holographic CARS imaging of sub-cellular components in live HeLa cells can be achieved.
相干反斯托克斯拉曼散射(CARS)全息术可捕获复反斯托克斯场的幅度和相位,并能通过数字聚焦到样本内部的不同深度来进行三维成像。展示了CARS全息术在生物成像中的应用。结果表明,可以实现对活的HeLa细胞中亚细胞成分的全息CARS成像。