北高加索多刺蔷薇果实的植物化学研究。

Phytochemical study of the north caucasian Rosa spinossima L. fruit.

作者信息

Novikov O O, Pisarev D I, Zhilyakova E T, Novikova M U, Bondarenko E V, Fadeeva D A, Bezmenova M D

机构信息

Belgorod State Medical University, Russia.

出版信息

Bull Exp Biol Med. 2011 Dec;152(2):216-8. doi: 10.1007/s10517-011-1492-8.

DOI:10.1007/s10517-011-1492-8

PMID:22808464

Abstract

Qualitative composition and content of anthocyanins in the Rosa spinossima L. fruit were studied. The anthocyanin sum was isolated by talc adsorption with subsequent desorption. The presence of anthocyanins in the resultant fraction was confirmed by thin layer chromatography. Three spots corresponding to anthocyanins were found. Anthocyanins were identified by mass spectrometry. Ion peaks corresponding to glycoside cyanidin and delphinidin were found. Position of sugar in the glycoside structure was determined by UV spectrophotometry with aluminum chloride. The content of anthocyanins was measured by direct UV spectrophotometry by the anthocyanins absorption. Anthocyanin content in raw material in conversion to cyanidin-3-glucoside was 1.46±0.03%.

摘要

对刺蔷薇果实中花色苷的定性组成和含量进行了研究。花色苷总量通过滑石吸附随后解吸进行分离。通过薄层色谱法确认了所得馏分中花色苷的存在。发现了三个与花色苷相对应的斑点。通过质谱法鉴定花色苷。发现了与糖苷矢车菊素和飞燕草素相对应的离子峰。通过氯化铝紫外分光光度法确定糖苷结构中糖的位置。通过花色苷吸收直接紫外分光光度法测量花色苷的含量。以矢车菊素-3-葡萄糖苷换算后，原料中花色苷的含量为1.46±0.03%。